Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula 72810, Puebla, Mexico.
Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Av. Gral. Ramón Corona No 2514, Colonia Nuevo México, Zapopan 45121, Jalisco, Mexico.
Biomolecules. 2024 Jun 30;14(7):782. doi: 10.3390/biom14070782.
In this work, the hexane, chloroform, and methanol extracts from were utilized to green-synthesize silver nanoparticles (Kf-, Kf-, and Kf-AgNPs). The Kf-, Kf-, and Kf-AgNPs were characterized by spectroscopy and microscopy techniques. The antibacterial activity of AgNPs was studied against bacteria strains, utilizing the microdilution assay. The DPPH and HO assays were considered to assess the antioxidant activity of AgNPs. The results revealed that Kf-, Kf-, and Kf-AgNPs exhibit an average diameter of 39.9, 111, and 42 nm, respectively. The calculated ζ-potential of Kf-, Kf-, and Kf-AgNPs were -20.5, -10.6, and -7.9 mV, respectively. The UV-vis analysis of the three samples demonstrated characteristic absorption bands within the range of 350-450 nm, which confirmed the formation of AgNPs. The FTIR analysis of AgNPs exhibited a series of bands from 3500 to 750 cm, related to the presence of extracts on their surfaces. SEM observations unveiled that Kf- and Kf-AgNPs adopted structural arrangements related to nano-popcorns and nanoflowers, whereas Kf-AgNPs were spherical in shape. It was determined that treatment with Kf-, Kf-, and Kf-AgNPs was demonstrated to inhibit the growth of , , and in a dose-dependent manner (50-300 μg/mL). Within the same range, treatment with Kf-, Kf-, and Kf-AgNPs decreased the generation of DPPH (IC 57.02-2.09 μg/mL) and HO (IC 3.15-3.45 μg/mL) radicals. This study highlights the importance of using inorganic nanomaterials to improve the biological performance of plant extracts as an efficient nanotechnological approach.
在这项工作中，利用 的正己烷、氯仿和甲醇提取物来绿色合成银纳米粒子（Kf-、Kf-和 Kf-AgNPs）。利用光谱和显微镜技术对 Kf-、Kf-和 Kf-AgNPs 进行了表征。利用微量稀释法研究了 AgNPs 对细菌菌株的抗菌活性。考虑了 DPPH 和 HO 测定法来评估 AgNPs 的抗氧化活性。结果表明，Kf-、Kf-和 Kf-AgNPs 的平均直径分别为 39.9、111 和 42nm。Kf-、Kf-和 Kf-AgNPs 的计算 ζ-电位分别为-20.5、-10.6 和-7.9mV。三种样品的 UV-vis 分析均在 350-450nm 范围内显示出特征吸收带，证实了 AgNPs 的形成。AgNPs 的 FTIR 分析显示出 3500 至 750cm 范围内的一系列谱带，与它们表面存在提取物有关。SEM 观察揭示了 Kf-和 Kf-AgNPs 采用与纳米爆米花和纳米花有关的结构排列，而 Kf-AgNPs 呈球形。结果表明，Kf-、Kf-和 Kf-AgNPs 的处理以剂量依赖的方式（50-300μg/mL）抑制 、 和 的生长。在相同范围内，Kf-、Kf-和 Kf-AgNPs 的处理降低了 DPPH（IC 57.02-2.09μg/mL）和 HO（IC 3.15-3.45μg/mL）自由基的生成。这项研究强调了使用无机纳米材料来提高植物提取物的生物性能作为一种有效的纳米技术方法的重要性。
