College of Plant Protection, Yunnan Agricultural University, Kunming 650224, China.
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Lab of Agricultural Biotechnology/Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650205, China.
Genes (Basel). 2024 Jul 16;15(7):924. doi: 10.3390/genes15070924.
The rice blight poses a significant threat to the rice industry, and the discovery of disease-resistant genes is a crucial strategy for its control. By exploring the rich genetic resources of Yuanjiang common wild rice () and analyzing their expression patterns, genetic resources can be provided for molecular rice breeding. The target genes' expression patterns, subcellular localization, and interaction networks were analyzed based on the annotated disease-resistant genes on the 9th and 10th chromosomes in the rice genome database using fluorescent quantitative PCR technology and bioinformatics tools. Thirty-three disease-resistant genes were identified from the database, including 20 on the 9th and 13 on the 10th. These genes were categorized into seven subfamilies of the NLR family, such as CNL and the G subfamily of the ABC family. Four genes were not expressed under the induction of the pathogen Y8, two genes were significantly down-regulated, and the majority were up-regulated. Notably, the expression levels of nine genes belonging to the ABCG, CN, and CNL classes were significantly up-regulated, yet the expression levels varied among roots, stems, and leaves; one was significantly expressed in the roots, one in the stems, and the remaining seven were primarily highly expressed in the leaves. Two interaction network diagrams were predicted based on the seven highly expressed genes in the leaves: complex networks regulated by CNL proteins and specific networks controlled by ABCG proteins. The disease-resistant genes on the 9th chromosome are actively expressed in response to the induction of rice blight, forming a critical gene pool for the resistance of Yuanjiang common wild rice () to rice blight. Meanwhile, the disease-resistant genes on the 10th chromosome not only participate in resisting the rice blight pathogen but may also be involved in the defense against other stem diseases.
稻瘟病对水稻产业构成重大威胁，而发现抗病基因是其防治的关键策略。通过探索元江普通野生稻丰富的遗传资源，并分析其表达模式，可以为分子水稻育种提供遗传资源。利用荧光定量 PCR 技术和生物信息学工具，对水稻基因组数据库中第 9 号和第 10 号染色体上注释的抗病基因进行目标基因表达模式、亚细胞定位和互作网络分析。从数据库中鉴定出 33 个抗病基因，其中第 9 号染色体 20 个，第 10 号染色体 13 个。这些基因分为 NLR 家族中的 7 个亚家族，如 CNL 和 ABC 家族的 G 亚家族。有 4 个基因在病原菌 Y8 的诱导下不表达，2 个基因显著下调，多数基因上调。值得注意的是，属于 ABCG、CN 和 CNL 类的 9 个基因的表达水平显著上调，但在根、茎和叶之间存在差异；一个在根中显著表达，一个在茎中表达，其余七个主要在叶中高度表达。根据叶中 7 个高表达基因预测了两个互作网络图：CNL 蛋白调控的复杂网络和 ABCG 蛋白控制的特异网络。第 9 号染色体上的抗病基因对稻瘟病的诱导反应活跃，形成元江普通野生稻（）对稻瘟病抗性的关键基因库。同时，第 10 号染色体上的抗病基因不仅参与抗稻瘟病病原菌，还可能参与抵抗其他茎部病害。
