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评估市售特级初榨橄榄油地理来源的分子溯源方法

Molecular Traceability Approach to Assess the Geographical Origin of Commercial Extra Virgin Olive Oil.

作者信息

Savoia Michele Antonio, Mascio Isabella, Miazzi Monica Marilena, De Giovanni Claudio, Grillo Spina Fabio, Carpino Stefania, Fanelli Valentina, Montemurro Cinzia

机构信息

Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy.

Department of the Central Inspectorate for the Protection of the Quality and Repression of Fraud of Food Products (ICQRF), Via Quintino Sella 42, 00187 Roma, Italy.

出版信息

Foods. 2024 Jul 16;13(14):2240. doi: 10.3390/foods13142240.

DOI:10.3390/foods13142240
PMID:39063323
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11275430/
Abstract

Extra virgin olive oil (EVOO) is a precious and healthy ingredient of Mediterranean cuisine. Due to its high nutritional value, the interest of consumers in the composition of EVOO is constantly increasing, making it a product particularly exposed to fraud. Therefore, there is a need to properly valorize high-quality EVOO and protect it from fraudulent manipulations to safeguard consumer choices. In our study, we used a straightforward and easy method to assess the molecular traceability of 28 commercial EVOO samples based on the use of SSR molecular markers. A lack of correspondence between the declared origin of the samples and the actual origin of the detected varieties was observed, suggesting possible adulteration. This result was supported by the identification of private alleles based on a large collection of national and international olive varieties and the search for them in the molecular profile of the analyzed samples. We demonstrated that the proposed method is a rapid and straightforward approach for identifying the composition of an oil sample and verifying the correspondence between the origin of olives declared on the label and that of the actual detected varieties, allowing the detection of possible adulterations.

摘要

特级初榨橄榄油(EVOO)是地中海美食中一种珍贵且健康的食材。由于其高营养价值,消费者对EVOO成分的关注度不断提高,这使得它成为一种特别容易遭受欺诈的产品。因此,有必要对高品质的EVOO进行合理评估,并保护其免受欺诈性操作,以保障消费者的选择。在我们的研究中,我们基于SSR分子标记的使用,采用了一种简单易行的方法来评估28个商业EVOO样品的分子可追溯性。观察到样品宣称的产地与检测到的品种的实际产地之间缺乏对应关系,这表明可能存在掺假情况。基于大量国内外橄榄品种的收集并在分析样品的分子图谱中寻找私有等位基因,这一结果得到了进一步支持。我们证明,所提出的方法是一种快速且直接的方法,可用于识别油样的成分,并验证标签上宣称的橄榄产地与实际检测到的品种的产地之间的对应关系,从而能够检测出可能的掺假情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/582e/11275430/4b39b92f8413/foods-13-02240-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/582e/11275430/68cf816b0d83/foods-13-02240-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/582e/11275430/b85e713f50ac/foods-13-02240-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/582e/11275430/4b39b92f8413/foods-13-02240-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/582e/11275430/68cf816b0d83/foods-13-02240-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/582e/11275430/b85e713f50ac/foods-13-02240-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/582e/11275430/4b39b92f8413/foods-13-02240-g003.jpg

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Nuclear magnetic resonance spectroscopy in extra virgin olive oil authentication.核磁共振光谱法在特级初榨橄榄油认证中的应用
Compr Rev Food Sci Food Saf. 2022 Sep;21(5):4056-4075. doi: 10.1111/1541-4337.13005. Epub 2022 Jul 25.
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The Relevance of Discovering and Recovering the Biodiversity of Apulian Almond Germplasm by Means of Molecular and Phenotypic Markers.
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Plants (Basel). 2022 Feb 21;11(4):574. doi: 10.3390/plants11040574.
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How to Choose a Good Marker to Analyze the Olive Germplasm ( L.) and Derived Products.如何选择合适的标志物分析橄榄种质资源(L.)及其衍生产品。
Genes (Basel). 2021 Sep 23;12(10):1474. doi: 10.3390/genes12101474.
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