Shih Wen-Ling, Yeh Tsung-Ming, Chen Kuang-Den, Leu Steve, Liu Shih-Feng, Huang Ying-Hsien, Kuo Ho-Chang
Department of Biological Science and Technology, National Pingtung University of Science and Technology, Neipu 912301, Taiwan.
General Research Service Center, National Pingtung University of Science and Technology, Neipu 912301, Taiwan.
Life (Basel). 2024 Jun 24;14(7):796. doi: 10.3390/life14070796.
Kawasaki disease (KD) is a syndrome primarily affecting young children, typically under the age of five, and is characterized by the development of acute vasculitis. Through extensive research conducted on both murine and human subjects, it has been demonstrated that heightened levels of reactive oxygen species (ROS) play a pivotal role in the development of KD, especial coronary artery lesions (CALs). Hydrogen gas exhibits potent antioxidant properties that effectively regulate ROS production and the inflammatory response.
We used cell wall extract (LCWE)-induced vasculitis in mice as an animal model of KD and treated the mice with hydrogen gas inhalation.
We observed significant dilatation and higher Z scores in the left coronary artery (LCA) in D21 and D28 in mice after LCWE treatment compared to the control group ( < 0.001) and a significant resolution of LCA diameters ( < 0.01) and Z scores ( < 0.01) after treatment with inhaled hydrogen gas. We further demonstrated that serum IL-6 expression was higher in mice after LCWE treatment ( < 0.01) and IL-6 significantly decreased after inhaled hydrogen gas therapy ( < 0.001).
According to our literature review, this is the first report where hydrogen gas inhalation has been demonstrated to be effective for the treatment of coronary artery dilatation in a KD murine model.
川崎病（KD）是一种主要影响幼儿（通常为五岁以下）的综合征，其特征是急性血管炎的发展。通过对小鼠和人类受试者进行的广泛研究，已证明活性氧（ROS）水平升高在KD的发展中起关键作用，尤其是冠状动脉病变（CALs）。氢气具有强大的抗氧化特性，可有效调节ROS的产生和炎症反应。
我们使用细胞壁提取物（LCWE）诱导的小鼠血管炎作为KD的动物模型，并对小鼠进行氢气吸入治疗。
与对照组相比，我们观察到LCWE治疗后第21天和第28天小鼠左冠状动脉（LCA）明显扩张且Z评分更高（P<0.001），吸入氢气治疗后LCA直径（P<0.01）和Z评分（P<0.01）显著恢复。我们进一步证明，LCWE治疗后小鼠血清IL-6表达更高（P<0.01），吸入氢气治疗后IL-6显著降低（P<0.001）。
根据我们的文献综述，这是首次报道吸入氢气被证明对KD小鼠模型中的冠状动脉扩张治疗有效。
