Zhang Shijie, Chen Ting, Chen Yu, Li Shucheng, Wang Wu, Zhao Yuqiang, Zhu Cancan
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), No. 1 Qianhuhoucun, Zhongshanmenwai, Xuanwu District, Nanjing 210014, China.
College of Agriculture, Anhui Science and Technology University, Fengyang, Chuzhou 233100, China.
Microorganisms. 2024 Jun 27;12(7):1313. doi: 10.3390/microorganisms12071313.
Pecan forests () are significant contributors to both food and oil production, and thrive in diverse soil environments, including coastal regions. However, the interplay between soil microbes and pecan forest health in coastal environments remains understudied. Therefore, we investigated soil bacterial and fungal diversity in coastal (Dafeng, DF) and inland (Guomei, GM) pecan plantations using high-throughput sequencing. The results revealed a higher microbial diversity in the DF plantation than in the GM plantation, significantly influenced by pH and edaphic factors. The dominant bacterial phyla were Proteobacteria, Acidobacteriota and Bacteroidota in the DF plantation, and Acidobacteriota, Proteobacteria, and Verrucomicrobiota in the GM plantation. , and were significantly more abundant bacterial genera in DF soil, whereas , and were more prevalent in GM soil. Basidiomycota dominated fungal sequences in the GM plantation, with a higher relative abundance of Ascomycota in the DF plantation. Significant differences in fungal genus composition were observed between plantations, with , , and being more abundant in DF soil, and , , and in GM soil. A functional analysis revealed greater carbohydrate metabolism potential in GM plantation bacteria and a higher ectomycorrhizal fungi abundance in DF soil. Significantly positive correlations were detected between certain bacterial and fungal genera and pH and total soluble salt content, suggesting their role in pecan adaptation to coastal environments and saline-alkali stress mitigation. These findings enhance our understanding of soil microbiomes in coastal pecan plantations, and are anticipated to foster ecologically sustainable agroforestry practices and contribute to coastal marshland ecosystem management.
山核桃林对粮食和油料生产都有重要贡献，并且能在包括沿海地区在内的多种土壤环境中茁壮成长。然而，沿海环境中山壤微生物与山核桃林健康之间的相互作用仍未得到充分研究。因此，我们利用高通量测序技术调查了沿海（大丰，DF）和内陆（国梅，GM）山核桃种植园的土壤细菌和真菌多样性。结果显示，DF种植园的微生物多样性高于GM种植园，这受到pH值和土壤因子的显著影响。DF种植园中主要的细菌门是变形菌门、酸杆菌门和拟杆菌门，GM种植园中是酸杆菌门、变形菌门和疣微菌门。 、 和 是DF土壤中显著更为丰富的细菌属，而 、 和 在GM土壤中更为普遍。担子菌门在GM种植园的真菌序列中占主导地位，DF种植园中子囊菌门的相对丰度更高。在不同种植园之间观察到真菌属组成存在显著差异， 、 和 在DF土壤中更为丰富， 、 和 在GM土壤中更为丰富。功能分析表明，GM种植园细菌中的碳水化合物代谢潜力更大，DF土壤中外生菌根真菌的丰度更高。在某些细菌和真菌属与pH值和总可溶性盐含量之间检测到显著的正相关，表明它们在山核桃适应沿海环境和缓解盐碱胁迫方面的作用。这些发现增进了我们对沿海山核桃种植园土壤微生物群落的理解，并有望促进生态可持续的农林业实践，为沿海沼泽地生态系统管理做出贡献。
