Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China.
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China.
Mol Plant. 2024 Sep 2;17(9):1392-1406. doi: 10.1016/j.molp.2024.07.011. Epub 2024 Jul 25.
Under warm temperatures, plants adjust their morphologies for environmental adaption via precise gene expression regulation. However, the function and regulation of alternative polyadenylation (APA), an important fine-tuning of gene expression, remains unknown in plant thermomorphogenesis. In this study, we found that SUMOylation, a critical post-translational modification, is induced by a long-term treatment at warm temperatures via a SUMO ligase SIZ1 in Arabidopsis. Disruption of SIZ1 altered the global usage of polyadenylation signals and affected the APA dynamic of thermomorphogenesis-related genes. CPSF100, a key subunit of the CPSF complex for polyadenylation regulation, is SUMOylated by SIZ1. Importantly, we demonstrated that SUMOylation is essential for the function of CPSF100 in genome-wide polyadenylation site choice during thermomorphogenesis. Further analyses revealed that the SUMO conjugation on CPSF100 attenuates its interaction with two isoforms of its partner CPSF30, increasing the nuclear accumulation of CPSF100 for polyadenylation regulation. In summary, our study uncovers a regulatory mechanism of APA via SIZ1-mediated SUMOylation in plant thermomorphogenesis.
在温暖的温度下，植物通过精确的基因表达调控来调整其形态以适应环境。然而，在植物热形态发生中，替代聚腺苷酸化（APA）的功能和调节——一种重要的基因表达精细调控——仍然未知。在这项研究中，我们发现在拟南芥中，SUMO 化，一种关键的翻译后修饰，通过 SUMO 连接酶 SIZ1 在长期的高温处理下被诱导。SIZ1 的破坏改变了多聚腺苷酸化信号的全局使用，并影响了与热形态发生相关基因的 APA 动态。CPSF100 是聚腺苷酸化调控的 CPSF 复合物的关键亚基，被 SIZ1 SUMO 化。重要的是，我们证明 SUMO 化对于 CPSF100 在热形态发生过程中全基因组多聚腺苷酸化位点选择中的功能是必不可少的。进一步的分析表明，CPSF100 上的 SUMO 缀合减弱了其与 CPSF30 的两个同工型的相互作用，增加了 CPSF100 的核积累以进行聚腺苷酸化调控。总之，我们的研究揭示了在植物热形态发生中，通过 SIZ1 介导的 SUMO 化来调节 APA 的调控机制。
