University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector 16-C Dwarka, New Delhi, 110078, India.
Protein J. 2024 Aug;43(4):923-934. doi: 10.1007/s10930-024-10222-z. Epub 2024 Jul 28.
Thrombosis is the formation of abnormal blood clots in the blood vessels that obstruct blood flow and lead to thrombosis. Current treatments for thrombosis are associated with serious side effects. Therefore there is a need for alternative natural therapy. A fibrinolytic protease was isolated from fresh leaves of Moringa oleifera Lam. and characterized for its potential to solubilize blood clots and hydrolyse fibrin under in-vitro conditions. The isolated protease showed a single protein band on native-PAGE. It showed optimum fibrinolytic activity at pH 8.0, 37 C with 50 µg protein. The fibrinolytic activity of isolated protease was also confirmed by fibrin zymography. K and V of isolated protease were determined by the Lineweaver Burk plot. The isolated protease could solubilize 96.41% of blood clots by 96 h under in-vitro conditions. In-vitro fibrin hydrolysis and blood clot solubilization activities shown by an isolated protease from leaves of Moringa oleifera Lam. suggest its fibrinolytic potential to dissolve blood clots. Being a natural molecule and from a dietary plant it can be explored as an alternative natural therapy against thrombosis.
血栓形成是指血管中异常血块的形成，会阻碍血液流动并导致血栓形成。目前治疗血栓的方法存在严重的副作用。因此，需要替代的天然疗法。从新鲜辣木叶中分离出一种纤维蛋白溶酶原激活物，并对其在体外条件下溶解血栓和水解纤维蛋白的潜力进行了特征描述。分离出的蛋白酶在天然聚丙烯酰胺凝胶电泳中显示出单一的蛋白质条带。它在 pH 值为 8.0、37°C、50µg 蛋白时表现出最佳的纤维蛋白溶酶活性。纤维蛋白溶酶活性也通过纤维蛋白凝胶电泳得到了证实。通过 Lineweaver Burk 作图确定了分离出的蛋白酶的 K 和 V 值。在体外条件下，分离出的蛋白酶在 96 小时内可溶解 96.41%的血栓。辣木叶分离出的蛋白酶具有体外纤维蛋白水解和血栓溶解活性，表明其具有溶解血栓的纤维蛋白溶酶潜力。作为一种天然分子和膳食植物来源的物质，它可以被探索作为一种替代的天然疗法来治疗血栓形成。
