Medical Laboratory Techniques, Vocational School of Health Services, Lokman Hekim University, Ankara, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey.
Biofouling. 2024 Sep;40(8):483-498. doi: 10.1080/08927014.2024.2381587. Epub 2024 Jul 29.
Using existing adrentimicrobials with essential oil components to prevent antimicrobial resistance is an alternative strategy. This study aimed to evaluate the resistance status, synergistic combinations, and biofilm formation activities of clinical isolates of methicillin-resistant (MRSA), and against antimicrobial agents and cinnamaldehyde, carvacrol, eugenol, limonene and eucalyptol. Antimicrobial activities were evaluated by microdilution, cytotoxicity by XTT, synergy by checkerboard and time-kill, and biofilm inhibition by microplate methods. Cinnamaldehyde and carvacrol showed strong antimicrobial activity. Synergistic effects were observed when using all essential oils with antimicrobials. Only two isolates showed antagonism with cinnamaldehyde and fluconazole. The constituents showed cytotoxic effects in the L929 cell line (except limonene). A time-kill analysis revealed a bacteriostatic effect on and MRSA isolates and a fungicidal effect on isolates. These results are important for further research to improve antimicrobial efficacy or to develop new agents.
利用含精油成分的现有抗生素来预防抗生素耐药性是一种替代策略。本研究旨在评估耐甲氧西林金黄色葡萄球菌（MRSA）和念珠菌临床分离株的耐药状况、协同组合和生物膜形成活性，以及对抗抗菌药物和肉桂醛、香芹酚、丁子香酚、柠檬烯和桉叶油醇的活性。采用微量稀释法评估抗菌活性，通过 XTT 评估细胞毒性，通过棋盘法和时间杀伤法评估协同作用，通过微孔板法评估生物膜抑制作用。肉桂醛和香芹酚表现出很强的抗菌活性。所有精油与抗生素联合使用时均观察到协同作用。只有两种分离株与肉桂醛和氟康唑表现出拮抗作用。这些成分在 L929 细胞系中表现出细胞毒性（除柠檬烯外）。时间杀伤分析显示对 和 MRSA 分离株有抑菌作用，对 分离株有杀菌作用。这些结果对于进一步研究提高抗菌疗效或开发新的药物非常重要。
