Restorative Dental Sciences Department, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Photobiomodul Photomed Laser Surg. 2024 Oct;42(10):634-642. doi: 10.1089/pho.2024.0077. Epub 2024 Jul 29.
To prepare experimental adhesive (EA) with 1% and without chitosan nanoparticles on dentin conditioned with a conventional technique phosphoric acid (PA) compared with two different contemporary techniques: photodynamic therapy (PDT) and femtosecond laser (FSL). The methodology consisted of synthesis of EA and 1% chitosan-modified adhesive (CMA). Scanning electron microscopy, dentin adhesive interface assessment, energy-dispersive spectroscopy, shear bond strength (SBS), degree of conversion (DC), and bond failure were assessed. Teeth were selected, disinfected, and mounted in acrylic up to the cementoenamel junction. Occlusal enamel was removed and teeth were randomly allocated into groups and conditioned. These included Group 1: samples treated with PA; Group 2: specimens conditioned with methylene blue photosensitizer (MBP) activated by PDT; and Group 3: samples conditioned with FSL. Following different conditioning regimes, specimens were bonded using 1% CMA and EA. The composite buildup was followed by SBS testing and a bond failure assessment. DC was assessed for both EA and CMA. Analysis of variance and Tukey's post hoc test were used to compare the mean and standard deviation of SBS and DC in different experimental groups, with a significance level of < 0.05. Dentin pretreated with etch and rinse demonstrated the highest bond strength with 1% CMA. Dentin conditioned with MBP activated by PDT and bonded to EA showed the lowest bond scores. Overall SBS values of 1% CMA were better than EA irrespective of the conditioning regime of dentin. The DC was higher in EA adhesive. This was followed by DC in 1% CMA. DC in EA was found to be comparable with 1% CMA. PA remains the gold standard for dentin conditioning. The incorporation of 1% chitosan in adhesive improves SBS and results in no change in DC. The use of FSL in dentin conditioning can be used as an alternative approach as it results in SBS within acceptable limits. The study was approved by the ethical board of King Saud University.
比较磷酸酸蚀（PA）、光动力疗法（PDT）和飞秒激光（FSL）三种不同当代技术预处理牙本质后，制备并评估 1%无壳聚糖纳米颗粒和含 1%壳聚糖修饰胶（CMA）的实验性黏结剂（EA）。
合成 EA 和 1%壳聚糖改性黏结剂（CMA）。采用扫描电子显微镜、牙本质黏结界面评估、能量色散光谱、剪切黏结强度（SBS）、转化率（DC）和黏结失败评估。选择、消毒牙齿，直至牙釉牙骨质界，用丙烯酸固定。去除咬合面釉质，将牙齿随机分为三组并进行预处理：第 1 组：用 PA 处理的样本；第 2 组：用亚甲蓝敏化剂（MBP）预处理，PDT 激活；第 3 组：用 FSL 预处理。在不同的预处理后，使用 1%CMA 和 EA 对样本进行黏结。在复合树脂堆积后进行 SBS 测试和黏结失败评估。评估 EA 和 CMA 的 DC。使用方差分析和 Tukey 事后检验比较不同实验组 SBS 和 DC 的平均值和标准差，显著性水平为 < 0.05。用 1%CMA 预处理牙本质的 SBS 值最高。用 PDT 激活 MBP 预处理牙本质并用 EA 黏结的 SBS 值最低。1%CMA 的总体 SBS 值优于 EA，无论牙本质的预处理方法如何。EA 黏结剂的 DC 较高，其次是 1%CMA。EA 的 DC 与 1%CMA 相当。PA 仍是牙本质预处理的金标准。黏结剂中添加 1%壳聚糖可提高 SBS，且对 DC 无影响。FSL 用于牙本质预处理时，可以作为一种替代方法，因为它可获得可接受范围内的 SBS。本研究得到了沙特国王大学伦理委员会的批准。
