Bi Yi, Lv Xiao, Wang Ke, Wu Jinyu, Shi Xiang, Zheng Xiaodong, Lin Xiaogang
Chongqing University Cancer Hospital, Chongqing University, Chongqing, China.
Key Laboratory of Optoelectronic Technology and Systems of Ministry of Education of China, Chongqing University, Chongqing, China.
Front Bioeng Biotechnol. 2024 Jul 12;12:1412598. doi: 10.3389/fbioe.2024.1412598. eCollection 2024.
Breast cancer currently stands as the most prevalent form of cancer worldwide and the primary cause of cancer-related deaths among women. However, the current diagnostic methods for breast cancer exhibit several limitations, including invasiveness, high costs, and limited sensitivity and specificity. The detection of the PIK3CA-H1047R variant is of paramount importance due to its close association with tumor growth and treatment resistance. Consequently, developing a straightforward, rapid, and highly sensitive approach for detecting PIK3CA-H1047R is of utmost importance. We have been working on the development of a rapid and ultrasensitive biosensor, leveraging the alternating current (AC) electrokinetic (ACEK) capacitive sensing method. This biosensor involves modifying the surface of interdigital electrodes with antibodies, facilitating the antibody-antigen-binding process through AC electrokinetic techniques. Our sensor strategy directly measures the interface capacitance, and the rate of change serves as a quantitative marker for event identification. Remarkably, our biosensor successfully detects the PIK3CA-H1047R antigen within a concentration range of 1 ng/mL to 1 μg/mL. In conclusion, this study proposes a fast and highly sensitive biosensor for the detection of a key breast cancer marker, the PIK3CA-H1047R variant. This technology is expected to improve breast cancer diagnosis, address the limitations of current methods, and provide patients with better treatment options. This detection method offers a promising avenue for on-site and real-time sensitive detection of the PIK3CA-H1047R antigen, potentially revolutionizing breast cancer diagnosis.
乳腺癌目前是全球最常见的癌症形式，也是女性癌症相关死亡的主要原因。然而，目前乳腺癌的诊断方法存在一些局限性，包括侵入性、高成本以及有限的灵敏度和特异性。PIK3CA-H1047R变体的检测至关重要，因为它与肿瘤生长和治疗耐药性密切相关。因此，开发一种直接、快速且高度灵敏的检测PIK3CA-H1047R的方法至关重要。我们一直致力于开发一种快速且超灵敏的生物传感器，利用交流（AC）电动（ACEK）电容传感方法。这种生物传感器通过用抗体修饰叉指电极表面，借助AC电动技术促进抗体-抗原结合过程。我们的传感器策略直接测量界面电容，电容变化率作为事件识别的定量标志物。值得注意的是，我们的生物传感器成功检测到浓度范围在1 ng/mL至1 μg/mL的PIK3CA-H1047R抗原。总之，本研究提出了一种用于检测关键乳腺癌标志物PIK3CA-H1047R变体的快速且高度灵敏的生物传感器。这项技术有望改善乳腺癌诊断，解决现有方法的局限性，并为患者提供更好的治疗选择。这种检测方法为PIK3CA-H1047R抗原的现场实时灵敏检测提供了一条有前景的途径，可能会彻底改变乳腺癌诊断。
