Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, People's Republic of China.
Key Laboratory of Shandong Microbial Engineering, College of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People's Republic of China.
Int J Nanomedicine. 2024 Jul 22;19:7399-7414. doi: 10.2147/IJN.S461866. eCollection 2024.
Influenza A (H1N1) virus is a highly contagious respiratory disease that causes severe illness and death. Vaccines and antiviral drugs are limited by viral variation and drug resistance, so developing efficient integrated theranostic options appears significant in anti-influenza virus infection.
In this study, we designed and fabricated covalent organic framework (COF) based theranostic platforms (T705@DATA-COF-Pro), which was composed of an RNA polymerase inhibitor (favipiravir, T705), the carboxyl-enriched COF (DATA-COF) nano-carrier and Cy3-labeled single DNA (ssDNA) probe.
The multi-porosity COF core provided an excellent micro-environment and smooth delivery for T705. The ssDNA probe coating bound to the nucleic acids of H1N1 selectively, thus controlling drug release and allowing fluorescence imaging. The combination of COF and probe triggered the synergism, promoting drug further therapeutic outcomes. With the aid of T705@DATA-COF-Pro platforms, the H1N1-infected mouse models lightly achieved diagnosis and significantly prolonged survival.
This research underscores the distinctive benefits and immense potential of COF materials in nano-preparations for virus infection, offering novel avenues for the detection and treatment of H1N1 virus infection.
甲型 H1N1 流感病毒是一种高度传染性的呼吸道疾病，可导致严重疾病和死亡。疫苗和抗病毒药物受到病毒变异和耐药性的限制，因此开发有效的综合治疗选择在抗流感病毒感染方面显得尤为重要。
在这项研究中，我们设计并制造了基于共价有机框架（COF）的治疗平台（T705@DATA-COF-Pro），它由 RNA 聚合酶抑制剂（法匹拉韦，T705）、富含羧基的 COF（DATA-COF）纳米载体和 Cy3 标记的单链 DNA（ssDNA）探针组成。
多孔隙 COF 核为 T705 提供了极好的微环境和顺畅的输送。ssDNA 探针涂层选择性地与 H1N1 的核酸结合，从而控制药物释放并允许荧光成像。COF 和探针的结合触发了协同作用，促进了药物的进一步治疗效果。在 T705@DATA-COF-Pro 平台的辅助下，感染 H1N1 的小鼠模型得到了轻度诊断，并显著延长了存活时间。
这项研究强调了 COF 材料在病毒感染纳米制剂中的独特优势和巨大潜力，为 H1N1 病毒感染的检测和治疗提供了新的途径。
