Guillouzo A, Begue J M, Campion J P, Gascoin M N, Guguen-Guillouzo C
Xenobiotica. 1985 Aug-Sep;15(8-9):635-41. doi: 10.3109/00498258509047421.
Viable adult human hepatocytes were obtained in large yields by perfusion of the liver of kidney donors. The hepatocytes were cultured either alone or in association with rat-liver epithelial cells. In pure culture the survival of hepatocytes did not exceed two to three weeks, while in co-culture they survived for several weeks and better retained the specific liver functions of albumin secretion, cytochrome P-450 content and glucuronidation of drugs. Human hepatocytes, particularly when mixed with rat-liver epithelial cells, may provide a valuable tool for predicting the metabolic pathways and hepatotoxicity of new drugs in man.
通过灌注肾供体的肝脏，可大量获得有活力的成人肝细胞。这些肝细胞单独培养或与大鼠肝脏上皮细胞联合培养。在纯培养中，肝细胞的存活时间不超过两到三周，而在共培养中，它们存活了数周，并且更好地保留了白蛋白分泌、细胞色素P - 450含量和药物葡萄糖醛酸化等特定肝脏功能。人肝细胞，特别是与大鼠肝脏上皮细胞混合时，可能为预测新药在人体内的代谢途径和肝毒性提供有价值的工具。
