Xue Jingwen, Zhou Zhipeng, Zhu Zhangrui, Sun Qi, Zhu Yuexuan, Wu Peng
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Department of Urology, Jinshan Branch of Fujian Provincial Hospital, Fuzhou, Fujian 350004, P.R. China.
Exp Ther Med. 2024 Jul 12;28(3):362. doi: 10.3892/etm.2024.12651. eCollection 2024 Sep.
Overactive bladder (OAB) is a condition characterized by an urgency to urinate, which is associated with the urodynamic observation of detrusor overexcitation. Although the etiology of OAB is currently unclear, it has been suggested that in patients with OAB, disruption of bladder epithelial barrier integrity can disturb the normal contractile function of the detrusor. Additionally, dietary preferences have been suggested to influence the severity of OAB. Therefore, the aim of the present study was to investigate the effect of a high salt diet (HSD) on the development of OAB in a murine model. Mice were fed either a HSD or standard diet for 8 weeks, following which voiding characteristics and bladder barrier function were assessed. The present study demonstrated that a HSD in mice was associated with OAB-like symptoms such as increased urinary frequency and non-voiding bladder contractions. The HSD group demonstrated a thinner bladder mucus layer and decreased expression of bladder barrier markers, tight junction protein-1 and claudin-1, which may be potentially indicative of induced bladder damage. A HSD for 8 weeks in mice and a high salt treatment at the uroepithelium cellular (SV-HUC-1s) level resulted in increased uroepithelial oxidative stress and inflammatory cell infiltration, as indicated by increased expression levels of TNF-α and IL-1β, as well as activation of the nucleotide-binding domain leucine-rich-containing family pyrin domain-containing 3 (NLRP3) and NF-κB signaling pathways and . Therefore, the present study indicated that a HSD could be a potentially important risk factor for the development of OAB, as it may be associated with overactivation of contractile function of the bladder by impairing the integrity of the bladder epithelial barrier and activation of the NLRP3 and NF-κB signaling pathways. Remodeling of the bladder barrier and reduction of the inflammatory response may be potential targets for the treatment of OAB in the future.
膀胱过度活动症（OAB）是一种以尿急为特征的病症，这与逼尿肌过度兴奋的尿动力学观察结果相关。尽管OAB的病因目前尚不清楚，但有人提出，在OAB患者中，膀胱上皮屏障完整性的破坏会干扰逼尿肌的正常收缩功能。此外，有人认为饮食偏好会影响OAB的严重程度。因此，本研究的目的是在小鼠模型中研究高盐饮食（HSD）对OAB发展的影响。给小鼠喂食HSD或标准饮食8周，之后评估排尿特征和膀胱屏障功能。本研究表明，小鼠的HSD与OAB样症状相关，如尿频增加和无排尿膀胱收缩。HSD组的膀胱黏液层较薄，膀胱屏障标志物紧密连接蛋白-1和闭合蛋白-1的表达降低，这可能潜在地表明膀胱受到了损伤。小鼠8周的HSD以及尿上皮细胞（SV-HUC-1s）水平的高盐处理导致尿上皮氧化应激增加和炎性细胞浸润，这表现为肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）表达水平升高，以及含核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）和核因子-κB（NF-κB）信号通路的激活。因此，本研究表明，HSD可能是OAB发展的一个潜在重要危险因素，因为它可能通过损害膀胱上皮屏障的完整性以及激活NLRP3和NF-κB信号通路而与膀胱收缩功能的过度激活有关。膀胱屏障重塑和炎症反应的减轻可能是未来治疗OAB的潜在靶点。
