Epigenetic landscape of intestinal cell line HT29 cocultured with .

To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify secretome. In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; HU protein interacts with histone H3. can epigenetically alter specific genetic pathways in human intestinal cells.