College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.
Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, People's Republic of China.
Mikrochim Acta. 2024 Jul 29;191(8):494. doi: 10.1007/s00604-024-06569-w.
Hyperproliferative diseases are the first step for tumor formation; thymidine kinase 1 (TK1) mRNA is closely related to cell proliferation. Therefore, the risk of malignant proliferation can be identified by sensitively detecting the variance in TK1 mRNA concentration, which can be used for tumor auxiliary diagnosis and monitoring tumor treatment. Owing to the low abundance and instability of TK1 mRNA in real samples, the development of a sensitive and fast mRNA detection method is necessary. A DNA nanosensor that can be used for detecting TK1 mRNA based on bipedal 3D DNA walker-driven proximal catalytic hairpin assembly (P-CHA) was developed. P-CHA hairpins were hybridized to a linker DNA strand coupled with magnetic nanoparticles to increase their local concentrations. The bipedal DNA walking on the surface of NPs accelerates reaction kinetics using the proximity effect. Taking advantage of the signal amplification of P-CHA as well as the rapid reaction rate of the DNA walker in 80 min, the proposed sensor detects TK1 mRNA with a low detection limit of 14 pM and may then be applied to clinical diagnosis.
过度增殖性疾病是肿瘤形成的第一步；胸苷激酶 1（TK1）mRNA 与细胞增殖密切相关。因此，通过敏感地检测 TK1 mRNA 浓度的变化，可以识别恶性增殖的风险，从而用于肿瘤辅助诊断和监测肿瘤治疗。由于真实样本中 TK1 mRNA 的丰度低且不稳定，因此有必要开发一种灵敏且快速的 mRNA 检测方法。开发了一种基于双足 3D DNA walker 驱动的近邻催化发夹组装（P-CHA）的 DNA 纳米传感器，可用于检测 TK1 mRNA。P-CHA 发夹与连接 DNA 链杂交，连接 DNA 链与磁性纳米颗粒偶联，以增加其局部浓度。双足 DNA 在 NPs 表面上的行走利用近邻效应加速反应动力学。利用 P-CHA 的信号放大以及 DNA walker 在 80 分钟内的快速反应速率，该传感器检测 TK1 mRNA 的检测限低至 14 pM，随后可应用于临床诊断。
