[转铁蛋白受体1和β1整合素的表达与乳腺癌雌激素受体状态及免疫浸润相关]

[Expression of transferrin receptor 1 and β1-integrins correlates with estrogen receptor status and immune infiltration in breast cancer].

作者信息

Chulkova S V, Sholokhova E N, Poddubnaya I V, Gladilina I A, Egorova A V, Stilidi I S

机构信息

N.N. Blokhin National Medical Research Center of Oncology, Moscow, Russia.

Pirogov Russian National Research Medical University, Moscow, Russia.

出版信息

Arkh Patol. 2024;86(4):23-30. doi: 10.17116/patol20248604123.

DOI:10.17116/patol20248604123

PMID:39073538

Abstract

UNLABELLED

Cancer cells can aberrantly express various markers, including transferrin receptor 1 (CD71) and 1-integrin molecules. Their role in invasion, migration and metastasis has been demonstrated. Determination of their expression in breast cancer (BC) may be an important point to characterize the clinical course of the tumor and prognosis of the disease.

OBJECTIVE

To study of transferrin receptor 1 (CD71) expression by primary breast cancer cells in correlation with tumor cell phenotype.

MATERIAL AND METHODS

Determination of BC phenotype: immunohistochemical staining method (immunofluorescence). Antibodies to ER (estrogen receptors), KL-1 (pancytokeratin), CD71 (transferrin receptor), CD29 (1-integrins). CD45, CD3, CD4, CD8, CD20 infiltration was also evaluated. ZEISS microscope (AXIOSKOP; Germany), method of G.J. Hammerling et al. Statistical processing: IBM-SPSS Statistics v.21.

RESULTS

63% of BC cases had CD71+ phenotype. CD71-mosaic tumors were observed in 14.4%. 1-integrin expression was monomorphic in 51.6% of cases and mosaic in 38.7%. 85% of ER-positive tumors were CD71-positive with a monomorphic type of reaction; =0.014. Among ER-negative tumors, CD71-negative reactions were 2-fold more frequent and the monomorphic type was less frequent. ER-positive tumors were CD29-positive in 73%; =0.031. 45.5% of ER+ tumors were CD29-monomorphic. Among ER-negative tumors, the frequency of CD29-monomorphic tumors was 55%. Significant infiltration by CD3+ cells was predominant in CD71-positive tumors; =0.016. In the CD29-monomorphic phenotype, CD45+ infiltration was 31.3%, and in the mosaic phenotype, 67.1%.

CONCLUSION

BC aberrantly expresses transferrin receptors, 1-integrins. CD71 expression is associated with ER expression. ER-positive tumors are often monomorphic for CD71. Prominent CD3+ infiltration was present in CD71+ tumors. Expression of 1-integrins correlated with ER+ status and weak immune infiltration.

摘要

未标记

癌细胞可异常表达多种标志物，包括转铁蛋白受体1（CD71）和α1整合素分子。它们在侵袭、迁移和转移中的作用已得到证实。检测它们在乳腺癌（BC）中的表达可能是表征肿瘤临床进程和疾病预后的重要依据。

目的

研究原发性乳腺癌细胞中转铁蛋白受体1（CD71）的表达与肿瘤细胞表型的相关性。

材料与方法

确定BC表型：免疫组织化学染色法（免疫荧光）。使用抗雌激素受体（ER）、全细胞角蛋白（KL-1）、CD71（转铁蛋白受体）、CD29（α1整合素）的抗体。还评估了CD45、CD3、CD4、CD8、CD20的浸润情况。采用ZEISS显微镜（AXIOSKOP；德国），G.J. Hammerling等人的方法。统计分析：IBM-SPSS Statistics v.21。

结果

63%的BC病例具有CD71+表型。观察到14.4%的病例为CD71镶嵌型肿瘤。51.6%的病例中α1整合素表达为单态性，38.7%为镶嵌型。85%的ER阳性肿瘤为CD71阳性，反应类型为单态性；P = 0.014。在ER阴性肿瘤中，CD71阴性反应的频率高出2倍，单态性类型较少见。73%的ER阳性肿瘤为CD29阳性；P = 0.031。45.5%的ER+肿瘤为CD29单态性。在ER阴性肿瘤中，CD29单态性肿瘤的频率为55%。CD71阳性肿瘤中CD3+细胞的显著浸润为主；P = 0.016。在CD29单态性表型中，CD45+浸润为31.3%，在镶嵌型表型中为67.1%。