School of Materials Science and Engineering, Zhengzhou University, Henan 450000, China.
School of Materials Science and Engineering, Zhengzhou University, Henan 450000, China.
Int J Biol Macromol. 2024 Oct;277(Pt 2):134222. doi: 10.1016/j.ijbiomac.2024.134222. Epub 2024 Jul 27.
In this work, carboxymethylated curdlan (CMCD) was utilized as a capping and stabilizing agent for the green synthesis of silver nanoparticles. Subsequently, quaternized curdlan (QCD) was introduced as the second capping layer through electrostatic attraction, leading to the preparation of double-capped silver nanoparticles (AgNPs@CQ). The successful synthesis of silver nanoparticles was characterized using UV-vis, FTIR, XRD, TEM, and DLS. AgNPs@CQ were incorporated into gelatin and a AgNPs@CQ/Gel composite hydrogel was obtained. The incorporation of AgNPs@CQ imparts excellent antibacterial properties to the composite hydrogel, thereby enhancing its antimicrobial efficacy. The presence of double-capping layers significantly retards the release rate of silver, contributing to prolonged antimicrobial activity. The MTT and live/dead fluorescence staining results demonstrate that the gelatin hydrogel incorporating double-capped AgNPs exhibits enhanced cell viability compared to the one incorporating single-capped AgNPs. Additionally, the composite hydrogel exhibits remarkable mechanical strength and adhesive performance. The AgNPs@CQ/Gel composite hydrogel demonstrates a cost-effective and facile preparation, showing significant potential in the field of dressings.
在这项工作中，羧甲基化的几丁聚糖（CMCD）被用作绿色合成银纳米粒子的封端和稳定剂。随后，通过静电吸引将季铵化的几丁聚糖（QCD）引入作为第二层封端剂，从而制备了双层包覆的银纳米粒子（AgNPs@CQ）。通过 UV-vis、FTIR、XRD、TEM 和 DLS 对银纳米粒子的成功合成进行了表征。AgNPs@CQ 被掺入明胶中，得到了 AgNPs@CQ/Gel 复合水凝胶。AgNPs@CQ 的掺入赋予了复合水凝胶优异的抗菌性能，从而提高了其抗菌效果。双层包覆显著延缓了银的释放速率，从而延长了抗菌活性。MTT 和活/死荧光染色结果表明，与掺入单包覆 AgNPs 的水凝胶相比，掺入双层包覆 AgNPs 的明胶水凝胶具有更高的细胞活力。此外，该复合水凝胶还表现出优异的机械强度和粘附性能。AgNPs@CQ/Gel 复合水凝胶具有成本效益和简单的制备方法，在敷料领域具有显著的应用潜力。
