Liu Xin, Bian Dan-Dan, Jiang Qi, Jiang Jun-Jie, Jin Ye, Chen Fan-Xing, Zhang Dai-Zhen, Liu Qiu-Ning, Tang Bo-Ping, Dai Li-Shang
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224007, People's Republic of China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, People's Republic of China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai 201306, People's Republic of China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224007, People's Republic of China; Anhui Key Laboratory of Resource Insect Biology and Innovative Utilization, College of Life Sciences, Anhui Agricultural University, Hefei 230036, People's Republic of China.
Int J Biol Macromol. 2024 Oct;277(Pt 2):134231. doi: 10.1016/j.ijbiomac.2024.134231. Epub 2024 Jul 27.
To investigate the impact of chlorantraniliprole on Procambarus clarkii, acute toxicity tests were performed. Results indicated that 96 h post-exposure to chlorantraniliprole (60 mg/L) led to the separation of the hepatopancreas basement membrane, causing cell swelling, rupture, and vacuolation. Moreover, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities exhibited divergent trends across four concentrations of chlorantraniliprole (0, 30, 60, and 90 mg/L). Hydrogen peroxide (HO) and catalase (CAT) levels significantly increased, while total superoxide dismutase (T-SOD) and malonaldehyde (MDA) activities decreased, indicating oxidative stress in the hepatopancreas. A total of 276 differentially expressed genes (DEGs) were identified, with 204 up-regulated and 72 down-regulated. Out of these, 114 DEGs were successfully annotated and classified into 99 pathways, with a primary focus on the cytochrome P450-mediated xenobiotic metabolism pathway. The DEGs enriched in this pathway, along with transcriptome data, were validated using quantitative-polymerase chain reaction. This study enhances the transcriptome database of P. clarkii and provides fundamental insights into its immune defense and antioxidant mechanisms. Additionally, it lays a theoretical foundation for future research on disease prevention in P. clarkii within rice-shrimp culture systems.
为研究氯虫苯甲酰胺对克氏原螯虾的影响，进行了急性毒性试验。结果表明，暴露于氯虫苯甲酰胺（60毫克/升）96小时后，肝胰腺基底膜分离，导致细胞肿胀、破裂和空泡化。此外，在氯虫苯甲酰胺的四种浓度（0、30、60和90毫克/升）下，酸性磷酸酶（ACP）和碱性磷酸酶（AKP）活性呈现出不同的趋势。过氧化氢（HO）和过氧化氢酶（CAT）水平显著升高，而总超氧化物歧化酶（T-SOD）和丙二醛（MDA）活性降低，表明肝胰腺存在氧化应激。共鉴定出276个差异表达基因（DEG），其中204个上调，72个下调。其中，114个DEG成功注释并归类为99条途径，主要集中在细胞色素P450介导的异生物质代谢途径。使用定量聚合酶链反应对该途径中富集的DEG以及转录组数据进行了验证。本研究增强了克氏原螯虾的转录组数据库，并为其免疫防御和抗氧化机制提供了基本见解。此外，它为未来在稻虾养殖系统中克氏原螯虾疾病预防研究奠定了理论基础。
