Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, 224007, PR China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, PR China.
Department of Infectious Disease, Ruian People's Hospital, Wenzhou, Zhejiang, 325200, PR China.
Fish Shellfish Immunol. 2019 Apr;87:43-50. doi: 10.1016/j.fsi.2018.12.055. Epub 2018 Dec 24.
Because of the high protein content and rich meat quality of crayfish Procambarus clarkii, it has become widely popular in China in recent years and has a high economic value. When P. clarkii is stimulated by heavy metals, it reacts to oxidation. P. clarkii has evolved antioxidant defense systems, including antioxidant enzymes such as catalase (CAT). The hexavalent form of Cr (VI) is a pathogenic factor that is of particular concern in aqueous systems because of its great toxicity to living organisms. In this study, we characterized the transcriptome of P. clarkii using a RNA sequencing method and performed a comparison between KCrO-treated samples and controls. In total, 34,237 unigenes were annotated. We identified 5098 significantly differentially expressed genes (DEGs), including 2536 and 2562 were significantly up-regulated and down-regulated, respectively. In addition, quantitative real time-PCR (qRT-PCR) confirmed the up-regulation of a random selection of DEGs. Our results contribute to a more comprehensive understanding of the antioxidant defense system used by P. clarkii in response to heavy metal stress.
由于小龙虾（Procambarus clarkii）的高蛋白含量和丰富的肉质，近年来在中国广受欢迎，具有很高的经济价值。当小龙虾受到重金属刺激时，它会发生氧化反应。小龙虾已经进化出抗氧化防御系统，包括过氧化氢酶（CAT）等抗氧化酶。六价铬（Cr（VI））是一种致病因素，因其对水生生物的高毒性而特别受到关注。在本研究中，我们使用 RNA 测序方法对小龙虾的转录组进行了表征，并对 KCrO 处理的样品和对照进行了比较。总共注释了 34237 个基因。我们鉴定了 5098 个显著差异表达基因（DEGs），其中分别有 2536 个和 2562 个基因显著上调和下调。此外，定量实时 PCR（qRT-PCR）验证了随机选择的 DEGs 的上调。我们的研究结果有助于更全面地了解小龙虾在应对重金属胁迫时使用的抗氧化防御系统。
