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在处理城市污水的高流速下流式悬挂式海绵反应器中存在独特的共氨氧化硝化螺旋菌。

Distinct comammox Nitrospira in high-rate down-flow hanging sponge reactor treating municipal wastewater.

机构信息

Department of Civil and Environmental Engineering, Nagaoka University of Technology, Niigata 940-2188, Japan.

Business Department, Global Business Division, NJS Co., LTD., Tokyo 105-0023, Japan.

出版信息

Bioresour Technol. 2024 Sep;408:131160. doi: 10.1016/j.biortech.2024.131160. Epub 2024 Jul 27.

DOI:10.1016/j.biortech.2024.131160
PMID:39074766
Abstract

A down-flow hanging sponge (DHS) reactor is a trickling filter system used for wastewater treatment, which employs sponges to retain biomass. This study assessed the process performance of a compact DHS combined with a sedimentation tank with seven phases at varying hydraulic retention times (HRT) over 500 days. The BOD of the DHS effluent was maintained at 4.0 ± 0.5 mg·L for the shortest HRT 0.3 ± 0.1 h. The nitrification efficiency was considerably impacted by the reduced HRT, with NH-N and NO-N concentrations of 9.0 ± 1.2 mgN·L and 2.2 ± 0.5 mgN·L, respectively. Nevertheless, the effluent complied with effluent discharge standards throughout the trial period. The number of comammox 16S rRNA gene copies ranged from 5.58 to 13.2 × 10 copies·mL, indicating that sponges biomass retained carrier can provide favorable conditions for comammox growth and could contribute to nitrification in the high-rate DHS reactor.

摘要

下向流悬挂式海绵(DHS)反应器是一种用于废水处理的滴滤滤池系统,采用海绵来保留生物量。本研究评估了紧凑 DHS 与沉淀槽结合使用的处理效果,在 500 多天的时间内,在不同水力停留时间(HRT)下进行了七个阶段的实验。在最短的 HRT 0.3±0.1 h 下,DHS 出水的 BOD 保持在 4.0±0.5 mg·L。硝化效率受到缩短 HRT 的显著影响,NH-N 和 NO-N 浓度分别为 9.0±1.2 mgN·L 和 2.2±0.5 mgN·L。然而,在整个试验期间,出水均符合排放标准。共氨氧化 16S rRNA 基因拷贝数范围为 5.58 到 13.2×10 拷贝·mL,表明海绵生物量保留载体可为共氨氧化生长提供有利条件,并有助于在高负荷 DHS 反应器中进行硝化。

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