Lin Qihua, Yang Yingying, Zhang Shusheng, Sun Faqian, Shen Chaofeng, Su Xiaomei
College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, 321004, China.
The Management Center of Wuyanling National Natural Reserve in Zhejiang, Wenzhou, 325500, China.
Environ Res. 2024 Nov 15;261:119699. doi: 10.1016/j.envres.2024.119699. Epub 2024 Jul 27.
The investigation into viable but non-culturable (VBNC) bacteria through the implementation of resuscitation promoting factors (Rpfs) has broadened the potential sources for isolating strains capable of degrading polychlorinated biphenyls (PCBs). Nonetheless, there has been limited research on the efficacy of resuscitated strains and the potential improvement of their performance through co-cultivation. In this work, the PCB degradation potential of resuscitated strains, specifically Pseudomonas sp. HR1 and Achromobacter sp. HR2, as well as their co-cultures, was investigated. Of particular importance was the comparative analysis between the optimal co-culture and individual strains regarding their ability to degrade PCB homologs and mineralize intermediate metabolites. The results suggested that the resuscitated strains HR1 and HR2 demonstrated robust growth and effective degradation of Aroclor 1242. The co-culture CO13, with an optimal HR1 to HR2 ratio of 1:3, exhibited a remarkable improvement in PCB degradation and intermediate metabolite mineralization compared to individual strains. Analysis of functional genes and degradation metabolites revealed that both the individual strains and co-culture CO13 degraded PCBs via the HOPDA-benzoate pathway, then mineralized through protocatechuate meta- and ortho-cleavage pathways, as well as the catechol ortho-cleavage pathway. This study represents the first documentation of the improved PCB degradation through the co-cultivation of resuscitated strains, which highlights the great promise of these resuscitated strains and their co-cultures as effective bio-inoculants for enhanced bioremediation.
通过实施复苏促进因子（Rpfs）对活的但不可培养（VBNC）细菌进行的研究，拓宽了分离能够降解多氯联苯（PCBs）菌株的潜在来源。尽管如此，关于复苏菌株的效能以及通过共培养对其性能进行潜在改善的研究仍然有限。在这项工作中，研究了复苏菌株，特别是假单胞菌属HR1和无色杆菌属HR2及其共培养物对多氯联苯的降解潜力。特别重要的是，对最佳共培养物和单个菌株在降解多氯联苯同系物和矿化中间代谢物能力方面进行了比较分析。结果表明，复苏菌株HR1和HR2表现出强劲的生长以及对Aroclor 1242的有效降解。HR1与HR2最佳比例为1:3的共培养物CO13，与单个菌株相比，在多氯联苯降解和中间代谢物矿化方面有显著改善。对功能基因和降解代谢物的分析表明，单个菌株和共培养物CO13均通过HOPDA-苯甲酸途径降解多氯联苯，然后通过原儿茶酸间位和邻位裂解途径以及儿茶酚邻位裂解途径进行矿化。这项研究首次记录了通过复苏菌株的共培养提高多氯联苯降解效果，这突出了这些复苏菌株及其共培养物作为增强生物修复有效生物接种剂的巨大潜力。
