Balasubramanian A
Stain Technol. 1979 Jul;54(4):177-80. doi: 10.3109/10520297909112680.
The exposed surface of plant tissues is coated with nail varnish. When peeled off, the transparent coating film bears a replica imprint of the tissue. Transparent adhesive tape is used for lifting the film imprint and mounting it on a microslide without curling. By a similar method, an opaque sealant replica with an aluminum foil support may be prepared which can serve as a mold for obtaining a transparent secondary imprint useful for light microscopic studies. The replicas can be stored like herbarium specimens for future use.
植物组织的暴露表面涂有指甲油。当指甲油被剥离时，透明的涂膜上会留下该组织的复制品印记。使用透明胶带提起薄膜印记并将其安装在载玻片上，使其不会卷曲。通过类似的方法，可以制备带有铝箔支撑的不透明密封剂复制品，该复制品可作为模具，用于获得对光学显微镜研究有用的透明二次印记。这些复制品可以像植物标本一样保存起来以备将来使用。
