Zhao Lin, Liu Yanghong, Chen Ye, Yu Zaixin, Luo Hui
Department of Cardiovascular Medicine, The Third Xiangya Hospital, Central South University, 410013 Changsha, Hunan, China.
Reproductive Medicine Center, The Third Xiangya Hospital, Central South University, 410013 Changsha, Hunan, China.
Rev Cardiovasc Med. 2024 Feb 18;25(2):67. doi: 10.31083/j.rcm2502067. eCollection 2024 Feb.
Ischaemia-reperfusion injury (IRI) is the damage that occurs when blood flow is restored to a tissue or organ after a period of ischaemia. Postconditioning is a therapeutic strategy aimed at reducing the tissue damage caused by IRI. Postconditioning in rodents is a useful tool to investigate the potential mechanisms of postconditioning. Currently, there is no convenient approach for postconditioning rodents.
Rats were subjected to a balloon postconditioning procedure. A balloon was used to control the flow in the vessel. This allowed for easy and precise manipulation of perfusion. Evans blue and triphenyltetrazolium chloride (TTC) double staining were used to determine the infarct size. Apoptosis in the myocardium was visualised and quantified by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). Western blotting was performed to assess the expression of key apoptotic proteins, , B-cell lymphoma 2 (Bcl-2), Bcl-2 Associated X (Bax), and cleaved caspase-3.
The balloon control approach to postconditioning provided accurate control of coronary blood flow and simplified the postconditioning manipulation. Infarct size reduction was observed in IRI rats after post-conditioning. There was a decrease in cardiac apoptosis in IRI rats after conditioning, as detected by TUNEL staining. IRI rats showed increased Bcl-2 levels and decreased Bax and cleaved caspase-3 levels in the myocardium.
Postconditioning was successfully applied in rats using this novel approach. Postconditioning with this approach reduced infarct size and apoptosis in the area at risk.
缺血再灌注损伤（IRI）是指组织或器官在经历一段时间的缺血后血流恢复时所发生的损伤。后适应是一种旨在减少IRI所致组织损伤的治疗策略。在啮齿动物中进行后适应是研究后适应潜在机制的有用工具。目前，尚无对啮齿动物进行后适应的便捷方法。
对大鼠进行球囊后适应操作。使用球囊控制血管内的血流。这使得灌注的操作简便且精确。采用伊文思蓝和氯化三苯基四氮唑（TTC）双重染色来确定梗死面积。通过末端脱氧核苷酸转移酶dUTP缺口末端标记法（TUNEL）对心肌细胞凋亡进行可视化和定量分析。进行蛋白质免疫印迹法以评估关键凋亡蛋白、B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）和裂解的半胱天冬酶-3的表达。
球囊控制后适应方法可精确控制冠状动脉血流并简化后适应操作。后适应后，IRI大鼠的梗死面积减小。TUNEL染色检测显示，后适应后IRI大鼠的心肌细胞凋亡减少。IRI大鼠心肌中的Bcl-2水平升高，Bax和裂解的半胱天冬酶-3水平降低。
采用这种新方法成功地在大鼠中实现了后适应。采用这种方法进行后适应可减小梗死面积并减少危险区域的细胞凋亡。
