Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
Bioresour Technol. 2024 Sep;408:131172. doi: 10.1016/j.biortech.2024.131172. Epub 2024 Jul 28.
Two protocols involving batch cultures were used to investigate the bioaugmentation of methane production by Pecoramyces ruminantium, and Methanobrevibacter thaueri. Protocol I examined the effect of altering the proportion of the microbial constituents in inoculum on alfalfa stalk fermentations and showed a 25 % improvement in dry matter loss in cultures where the inoculum contained just 30 % of co-culture and 70 % of fungal monoculture. Protocol II involved consecutive cultures and alternating inoculations. This protocol resulted in 17-22 mL/g DM methane production with co-cultures a 30 % increase in methane relative to the fungal monoculture. Both protocols indicate that the co-culture rapidly dominated and was more resilient than the monoculture. Synergistic interaction between fungus and methanogen, promoted more efficient lignocellulose degradation and higher methane yield. This study highlighted the potential of microbial co-cultures for enhancing methane production from lignocellulosic biomass, offering a promising bioaugmentation strategy for improving biogas yields and waste valorization.
采用两种批次培养方案研究了瘤胃新月形单胞菌（Pecoramyces ruminantium）和产甲烷短杆菌（Methanobrevibacter thaueri）对甲烷生产的生物强化作用。方案 I 考察了改变接种物中微生物组成比例对苜蓿秸秆发酵的影响，结果表明，在接种物中仅包含 30%共培养物和 70%真菌纯培养物的培养物中，干物质损失提高了 25%。方案 II 涉及连续培养和交替接种。该方案使共培养物的甲烷产量达到 17-22 mL/g DM，与真菌纯培养物相比，甲烷产量增加了 30%。这两个方案都表明，共培养物迅速占据主导地位，比纯培养物更具弹性。真菌和产甲烷菌之间的协同相互作用促进了木质纤维素的更有效降解和更高的甲烷产量。本研究强调了微生物共培养物在提高木质纤维素生物质甲烷生产中的潜力，为提高沼气产量和废物增值提供了一种有前途的生物强化策略。
