B 细胞表位作图：迈向更好的疫苗和治疗性抗体。

B cell epitope mapping: The journey to better vaccines and therapeutic antibodies.

机构信息

Curtin Medical School, Curtin University, Western Australia, Australia; Curtin Health Innovation Research Institute, Curtin University, Western Australia, Australia.

Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Western Australia, Australia; Centre for Child Health Research, The University of Western Australia, Western Australia, Australia; Department of Clinical Immunology, PathWest Laboratory Medicine, Western Australia, Australia.

出版信息

Biochim Biophys Acta Gen Subj. 2024 Oct;1868(10):130674. doi: 10.1016/j.bbagen.2024.130674. Epub 2024 Jul 28.

DOI:10.1016/j.bbagen.2024.130674

PMID:39079649

Abstract

B-cell epitope mapping is an approach that can identify and characterise specific antigen binding sites of B-cell receptors and secreted antibodies. The ability to determine the antigenic clusters of amino acids bound by B-cell clones provides unprecedented detail that will aid in developing novel and effective vaccine targets and therapeutic antibodies for various diseases. Here, we discuss conventional approaches and emerging techniques that are used to map B-cell epitopes.

摘要

B 细胞表位作图是一种能够识别和描述 B 细胞受体和分泌型抗体特定抗原结合位点的方法。确定 B 细胞克隆结合的抗原簇的氨基酸的能力提供了前所未有的细节，这将有助于开发针对各种疾病的新型有效疫苗靶点和治疗性抗体。在这里，我们讨论了用于绘制 B 细胞表位的常规方法和新兴技术。