National Key Laboratory of Green Pesticide, Guangzhou 510642, China.
Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China.
J Agric Food Chem. 2024 Aug 14;72(32):17858-17867. doi: 10.1021/acs.jafc.4c03530. Epub 2024 Jul 31.
In , both males and females release chemical signals to attract mates. In our previous study, we identified ethyl laurate, ethyl myristate, and ethyl palmitate as potent female-derived pheromones that contribute to mate attraction. However, the mechanisms underlying the olfactory recognition remain unclear. In this study, we observed strong antennal and behavioral responses in male to these female-derived pheromones, and further investigation revealed significant upregulation of and following exposure to these compounds. Through fluorescence competitive binding assays and RNA interference techniques, we demonstrated the crucial roles of OBP49a and OBP83b in detecting female-derived pheromones. Finally, molecular docking analysis identified key residues, including His134 in OBP83b and a lysine residue in OBP49a, which formed hydrogen bonds with female-derived pheromones, facilitating their binding. These findings not only advance our understanding of olfactory recognition of pheromones in but also offer potential targets for developing olfaction-interfering techniques for pest control.
在昆虫中，雄性和雌性都会释放化学信号来吸引配偶。在我们之前的研究中，我们鉴定出月桂酸乙酯、肉豆蔻酸乙酯和棕榈酸乙酯是具有很强吸引力的雌性衍生信息素。然而，嗅觉识别的机制仍不清楚。在这项研究中，我们观察到雄性对这些雌性衍生信息素表现出强烈的触角和行为反应，进一步的研究表明，这些化合物暴露后， 和 显著上调。通过荧光竞争结合测定和 RNA 干扰技术，我们证明了 OBP49a 和 OBP83b 在检测雌性衍生信息素中的关键作用。最后，分子对接分析确定了关键残基，包括 OBP83b 中的 His134 和 OBP49a 中的一个赖氨酸残基，它们与雌性衍生信息素形成氢键，促进其结合。这些发现不仅增进了我们对 中信息素嗅觉识别的理解，也为开发用于害虫防治的嗅觉干扰技术提供了潜在的目标。
