Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Hefei, Anhui, China.
Institute of Dermatology, Anhui Medical University, Hefei, Anhui, China.
Front Immunol. 2024 Jul 16;15:1401102. doi: 10.3389/fimmu.2024.1401102. eCollection 2024.
Immune-related skin diseases represent a collective of dermatological disorders intricately linked to dysfunctional immune system processes. These conditions are primarily characterized by an immoderate activation of the immune system or deviant immune responses, involving diverse immune components including immune cells, antibodies, and inflammatory mediators. However, the precise molecular dysregulation underlying numerous individual cases of these diseases and unique subsets respond under disease conditions remains elusive. Comprehending the mechanisms and determinants governing the homeostasis and functionality of diseases could offer potential therapeutic opportunities for intervention. Mass cytometry enables precise and high-throughput quantitative measurement of proteins within individual cells by utilizing antibodies labeled with rare heavy metal isotopes. Imaging mass cytometry employs mass spectrometry to obtain spatial information on cell-to-cell interactions within tissue sections, simultaneously utilizing more than 40 markers. The application of single-cell mass cytometry presents a unique opportunity to conduct highly multiplexed analysis at the single-cell level, thereby revolutionizing our understanding of cell population heterogeneity and hierarchy, cellular states, multiplexed signaling pathways, proteolysis products, and mRNA transcripts specifically in the context of many autoimmune diseases. This information holds the potential to offer novel approaches for the diagnosis, prognostic assessment, and monitoring responses to treatment, thereby enriching our strategies in managing the respective conditions. This review summarizes the present-day utilization of single-cell mass cytometry in studying immune-related skin diseases, highlighting its advantages and limitations. This technique will become increasingly prevalent in conducting extensive investigations into these disorders, ultimately yielding significant contributions to their accurate diagnosis and efficacious therapeutic interventions.
免疫相关性皮肤疾病是一组与免疫系统功能障碍密切相关的皮肤科疾病。这些疾病的主要特征是免疫系统过度激活或免疫反应异常，涉及多种免疫成分，包括免疫细胞、抗体和炎症介质。然而，许多这类疾病的个体病例以及在疾病条件下出现的独特亚群背后的精确分子失调仍然难以捉摸。理解疾病的稳态和功能的机制和决定因素可能为干预提供潜在的治疗机会。
质谱流式细胞术通过利用标记稀有重金属同位素的抗体，能够精确和高通量地测量单个细胞内的蛋白质。成像质谱流式细胞术利用质谱技术获取组织切片中细胞间相互作用的空间信息，同时利用超过 40 种标记物。单细胞质谱流式细胞术的应用提供了在单细胞水平上进行高度多重分析的独特机会，从而彻底改变了我们对细胞群体异质性和层次、细胞状态、多重信号通路、蛋白水解产物和特定于许多自身免疫性疾病的 mRNA 转录本的理解。这些信息有可能为诊断、预后评估和治疗反应监测提供新的方法，从而丰富我们管理这些疾病的策略。
本综述总结了单细胞质谱流式细胞术在研究免疫相关性皮肤疾病中的当前应用，强调了其优点和局限性。这项技术将越来越多地用于对这些疾病进行广泛研究，最终对这些疾病的准确诊断和有效的治疗干预做出重大贡献。
