First Department of Propaedeutic Internal Medicine and Joint Rheumatology Program, School of Medicine, National and Kapodistrian University of Athens, Athens 11527, Greece; School of Infection and Immunity, University of Glasgow, Glasgow, UK.
Centre of New Biotechnologies and Precision Medicine (CNBPM), School of Medicine, National and Kapodistrian University of Athens, Athens 11527, Greece.
Clin Immunol. 2023 Aug;253:109679. doi: 10.1016/j.clim.2023.109679. Epub 2023 Jun 17.
Mass cytometry was employed to investigate 47 circulating leukocyte subsets in patients with active psoriatic arthritis (PsA, n = 16) compared to healthy controls (n = 13), seropositive (RF and/or anti-CCP, n = 12) and seronegative (n = 9) RA patients. Comparing PsA to controls, different cell frequencies were found in both innate and adaptive immunity cell subsets, as well as in cells bridging innate and adaptive immunity. In some T cell subsets increased costimulatory molecules' expression in PsA, was also noted.No changes were observed in patients who remained disease-active after 3 months of treatment, in contrast to those who achieved remission/low-disease activity. Comparing PsA to seropositive RA, elevated frequencies of naïve and activated CD8 T cells, B cells, MAIT/iNKT and ILCs were found, while the opposite was the case for terminal effector, senescent, and Th2-like cells. Strikingly, the composition of the leukocyte pool in PsA was comparable to seronegative RA, providing evidence for the pathogenetic similarities between these two entities.
采用液质联用技术分析了 16 例活动性银屑病关节炎（PsA）患者、13 例健康对照者、12 例血清阳性（RF 和/或抗 CCP）和 9 例血清阴性（RF 和/或抗 CCP）类风湿关节炎（RA）患者的 47 种循环白细胞亚群。与对照组相比，在固有免疫和适应性免疫细胞亚群以及连接固有免疫和适应性免疫的细胞中，均发现了不同的细胞频率。在一些 T 细胞亚群中，还观察到 PsA 中协同刺激分子表达增加。与治疗 3 个月后仍处于疾病活动状态的患者相比，达到缓解/低疾病活动度的患者无变化。与血清阳性 RA 相比，在 PsA 中发现了幼稚和活化的 CD8 T 细胞、B 细胞、MAIT/iNKT 和 ILC 的频率升高，而终末效应、衰老和 Th2 样细胞的频率则相反。值得注意的是，PsA 中白细胞池的组成与血清阴性 RA 相似，为这两种疾病实体之间存在相似的发病机制提供了证据。
