Kohchiyama A, Hatamochi A, Ueki H
Dermatologica. 1985;171(5):327-31. doi: 10.1159/000249446.
In 6 patients with untreated alopecia areata in the progressive stage, 6 in the stationary stage, and 6 normal individuals as controls, an in situ analysis of OKT6-positive dendritic cells in hair follicles, and peribulbar and intrabulbar infiltrates was performed using the avidin-biotin-peroxidase method with monoclonal antibodies. In controls, OKT6-positive dendritic cells were distributed only in the upper portions of hair follicles and were not observed in the bulbar area, and the percentage of these cells among all epithelial cells of the hair follicles was 1.0 +/- 0.1% (mean +/- SE). In stationary-stage patients, the distribution and the percentage of positive cells were the same as those for the controls (1.1 +/- 0.1%). In the progressive stage, however, positive cells were distributed in both the upper portions of the hair follicles and the bulbar area, and the percentage of positive cells (4.9 +/- 0.3%) was significantly higher than that of controls. Staining for T, B lymphocytes and T cell subsets in the peribulbar infiltrates revealed a predominance of OKT4-positive cells (the OKT4/OKT8 ratio was from 3:1 to 4:1). This indicates that the number of OKT6-positive dendritic cells increases in the hair follicles of progressive alopecia areata and that these cells may play an important role in cooperation with T cells in the pathogenesis of alopecia areata.
对6例处于进行期的未经治疗的斑秃患者、6例静止期患者以及6名作为对照的正常个体，采用抗生物素蛋白-生物素-过氧化物酶法及单克隆抗体，对毛囊、球周及球内浸润中的OKT6阳性树突状细胞进行原位分析。在对照组中，OKT6阳性树突状细胞仅分布于毛囊上部，球部未观察到，这些细胞在毛囊所有上皮细胞中的百分比为1.0±0.1%（均值±标准误）。在静止期患者中，阳性细胞的分布及百分比与对照组相同（1.1±0.1%）。然而，在进行期，阳性细胞分布于毛囊上部及球部，阳性细胞百分比（4.9±0.3%）显著高于对照组。对球周浸润中的T、B淋巴细胞及T细胞亚群染色显示，OKT4阳性细胞占优势（OKT4/OKT8比值为3:1至4:1）。这表明进行期斑秃患者毛囊中OKT6阳性树突状细胞数量增加，且这些细胞在斑秃发病机制中可能与T细胞协同发挥重要作用。
