Camargo Cristina Pires, Santos Deborah Luisa de Sousa, Cerqueira Dantas Viviane Abreu Nunes, Furuya Tatiane Katsue, Freitas-Marchi Bruna Leticia, Alves Maria José Ferreira, Uno Miyuki, Gemperli Rolf
From the Microsurgery and Plastic Surgery Laboratory, School of Medicine, Universidade de São Paulo, São Paulo, SP, Brazil.
Multiprofessional Residency Program in Oncology Care for Adults, Comissão de Residência Multiprofissional (COREMU), Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil.
Plast Reconstr Surg Glob Open. 2024 Jul 30;12(7):e5977. doi: 10.1097/GOX.0000000000005977. eCollection 2024 Jul.
Capsular contracture is a common complication affecting about 80% of patients who receive radiotherapy after breast reconstruction with silicone prostheses. This study examines the use of adipocyte stem cells (ASCs) to treat capsular contracture.
Thirty rats were operated on to implant a minisilicone prosthesis in the dorsal region. The rats were divided into three groups: control (saline solution injection), radiotherapy (RDT), and RDT + ASC. After 3 months, the capsules were collected and submitted to histological analysis for inflammatory cell presence, vascular density, and collagen fibers, and gene expression of Tnf, , , , , and by qPCR.
In macroscopic analysis, the RTGO score showed a two-point reduction in RDT + ASC compared with the RDT ( = 0.003). In histological analysis, ASC exhibited less than 50% of inflammatory cells compared with RDT ( = 0.004), which was similar to control. This study demonstrated that gene expression was identical in both RDT and RTD + ASC. Compared with control, treatment with ASC reduced expression by 30%. and expression levels were similar in both the control and RTD + ASC.
This study suggests that ASC treatment decreases silicone prosthesis capsule inflammation.
包膜挛缩是一种常见并发症，影响约80%接受硅胶假体乳房重建后放疗的患者。本研究探讨脂肪干细胞（ASC）用于治疗包膜挛缩的效果。
对30只大鼠进行手术，在其背部植入微型硅胶假体。大鼠被分为三组：对照组（注射生理盐水）、放疗组（RDT）和放疗+ASC组。3个月后，收集包膜并进行组织学分析，以检测炎症细胞的存在、血管密度和胶原纤维，并通过qPCR检测Tnf、 、 、 、 和 的基因表达。
在宏观分析中，RTGO评分显示放疗+ASC组比放疗组降低了两分（ = 0.003）。在组织学分析中，与放疗组相比，ASC组炎症细胞减少了50%以上（ = 0.004），与对照组相似。本研究表明，放疗组和放疗+ASC组的 基因表达相同。与对照组相比，ASC治疗使 表达降低了30%。对照组和放疗+ASC组的 和 表达水平相似。
本研究表明ASC治疗可减轻硅胶假体包膜炎症。
