遗传性结肠腺癌患者培养的皮肤成纤维细胞中肌动蛋白组织缺陷，添加纤连蛋白后也无法恢复。

Defective actin organization in cultured skin fibroblasts from individuals with inherited colon adenocarcinoma is not restored by addition of fibronectin.

作者信息

Kopelovich L, Murray J C, Kleinman H K, Martin G R, Fukuda Y, Rennard S I

出版信息

Exp Cell Biol. 1985;53(6):314-21. doi: 10.1159/000163328.

Abstract

Cultured skin fibroblasts from patients with hereditary adenomatosis of the colon and rectum (ACR) have a disorganized cytoskeleton. Since fibronectin has been found to restore normal cytoskeletal and cellular morphology to transformed cells, we investigated the fibronectin pattern in ACR cells. We found that although both fibronectin synthesis and binding to the cell surface were apparently normal, addition of fibronectin did not restore a normal distribution of actin cables to ACR cells. The data suggest that coupling of cell surface-bound fibronectin to the cytoskeleton might be constitutively defective in ACR cells.

摘要

来自遗传性结肠直肠癌（ACR）患者的培养皮肤成纤维细胞具有紊乱的细胞骨架。由于已发现纤连蛋白可使转化细胞恢复正常的细胞骨架和细胞形态，我们研究了ACR细胞中的纤连蛋白模式。我们发现，尽管纤连蛋白的合成以及与细胞表面的结合显然正常，但添加纤连蛋白并未使ACR细胞中肌动蛋白丝束恢复正常分布。数据表明，在ACR细胞中，细胞表面结合的纤连蛋白与细胞骨架的偶联可能存在先天性缺陷。