Gui Xinyue, Li Fei, Cui Xinyu, Wu Ranran, Liu Dingyu, Ma Chunling, Ma Lijuan, Jiang Huifeng, You Chun, Zhu Zhiguang
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, The College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Biodes Res. 2024 Jul 30;6:0039. doi: 10.34133/bdr.0039. eCollection 2024.
Terpenoids of substantial industrial interest are mainly obtained through direct extraction from plant sources. Recently, microbial cell factories or in vitro enzymatic biosystems have emerged as promising alternatives for terpenoid production. Here, we report a route for the synthesis of α-farnesene based on an in vitro enzyme cascade reaction using methanol as an inexpensive and renewable C1 substrate. Thirteen biocatalytic reactions divided into 2 modules were optimized and coupled to achieve methanol-to-α-farnesene conversion via integration with natural thylakoid membranes as a green energy engine. This in vitro enzymatic biosystem driven by light enabled the production of 1.43 and 2.40 mg liter α-farnesene using methanol and the intermediate glycolaldehyde as substrates, respectively. This work could provide a promising strategy for developing light-powered in vitro biosynthetic platforms to produce more natural compounds synthesized from C1 substrates.
具有重大工业价值的萜类化合物主要通过从植物来源直接提取获得。最近，微生物细胞工厂或体外酶促生物系统已成为萜类化合物生产的有前景的替代方法。在此，我们报告了一种基于体外酶级联反应合成α-法尼烯的路线，该反应使用甲醇作为廉价且可再生的C1底物。将13个生物催化反应分为2个模块进行优化并耦合，通过与作为绿色能源引擎的天然类囊体膜整合，实现了甲醇到α-法尼烯的转化。这种由光驱动的体外酶促生物系统分别使用甲醇和中间产物乙醇醛作为底物，能够生产1.43和2.40毫克/升的α-法尼烯。这项工作可为开发光驱动的体外生物合成平台以生产更多由C1底物合成的天然化合物提供一种有前景的策略。
