Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Microb Drug Resist. 2024 Sep;30(9):385-390. doi: 10.1089/mdr.2024.0070. Epub 2024 Jul 31.
Emerging resistance of Gram-negative bacteria, including , to commonly used detergents and disinfectant is encountering us with hazard. Inappropriate use of disinfectants has forced bacteria to gain resistance. The ability of bacteria to extrude substrates from the cellular interior to the external environment has enabled them to persist in exposure to toxic compounds, which is due to existence of transport proteins. Efflux pumps, in Gram-negative bacteria, are proteins responsible for exporting molecules outside of the cell, by crossing the two membranes. In this study, 40 strains from hospitals, clinics, and burn center laundries and 40 strains from urban laundries were collected. This study evaluated the minimum inhibitory concentration (MIC) level of sodium dodecyl sulfate (SDS), didecyldimethylammonium chloride (DDAC), and octenidine dihydrochloride (Od) in strains. The real-time PCR was carried out to evaluate the expression of MexAB-OprM, MexCD-OprJ, and MexXY-OprM efflux system. The obtained results indicated a higher MIC level for SDS, DDAC, and Od in medical laundries. The sub-MIC level of DDAC and Od increased the expression level of MexAB-OprM, MexCD-OprJ, and MexXY-OprM in strains, suggesting that efflux pumps contribute to disinfectant resistance in .
革兰氏阴性菌（包括 ）对抗生素和消毒剂的耐药性日益增强，这给我们带来了极大的危害。消毒剂的不当使用迫使细菌产生了耐药性。细菌具有将细胞内的底物排出到外部环境中的能力，这使它们能够在暴露于有毒化合物时存活下来，这是由于存在转运蛋白。在革兰氏阴性菌中，外排泵是负责将分子从细胞内排出到细胞外的蛋白质，通过穿过两层膜。在这项研究中，从医院、诊所和烧伤中心的洗衣房收集了 40 株菌株，从城市洗衣房收集了 40 株菌株。本研究评估了 40 株 菌株中十二烷基硫酸钠（SDS）、双十二烷基二甲基氯化铵（DDAC）和奥替尼啶二盐酸盐（Od）的最小抑菌浓度（MIC）水平。采用实时 PCR 技术评估 MexAB-OprM、MexCD-OprJ 和 MexXY-OprM 外排系统的表达。结果表明，医疗洗衣房中 SDS、DDAC 和 Od 的 MIC 水平较高。DDAC 和 Od 的亚 MIC 水平增加了 株菌中 MexAB-OprM、MexCD-OprJ 和 MexXY-OprM 的表达水平，表明外排泵有助于 对消毒剂的耐药性。
