Department of Anatomy and Cell Biology, The George Washington University School of Medicine and Health Sciences, Washington, DC 20052, USA.
The George Washington University Cancer Center, The George Washington University School of Medicine and Health Sciences, Washington, DC 20052, USA.
Development. 2024 Aug 15;151(16). doi: 10.1242/dev.203070. Epub 2024 Aug 29.
Tissue-resident macrophages contribute to the organogenesis of many tissues. Growth of the prostate is regulated by androgens during puberty, yet androgens are considered immune suppressive. In this study, we characterized the localization, androgen receptor expression and hematopoietic origin of prostate macrophages, and transiently ablated macrophages during postnatal prostate organogenesis in the mouse. We show that myeloid cells were abundant in the prostate during puberty. However, nuclear androgen receptor expression was not detected in most macrophages. We found Cx3cr1, a marker for macrophages, monocytes and dendritic cells, expressed in interstitial macrophages surrounding the prostate and associated with nerve fibers. Furthermore, we provide evidence for the co-existence of embryonic origin, self-renewing, tissue-resident macrophages and recruited macrophages of bone-marrow monocyte origin in the prostate during puberty. Our findings suggest that prostate macrophages promote neural patterning and may shed further light on our understanding of the role of the innate immune system in prostate pathology in response to inflammation and in cancer.
组织驻留巨噬细胞有助于许多组织的器官发生。前列腺的生长在青春期受雄激素调节，但雄激素被认为具有免疫抑制作用。在这项研究中，我们描述了前列腺巨噬细胞的定位、雄激素受体表达和造血来源，并在小鼠的出生后前列腺器官发生期间短暂地消除了巨噬细胞。我们发现，在青春期，前列腺中有大量的髓样细胞。然而，大多数巨噬细胞中未检测到核雄激素受体表达。我们发现 Cx3cr1，一种用于标记巨噬细胞、单核细胞和树突状细胞的标志物，在围绕前列腺的间质巨噬细胞中表达，并与神经纤维相关。此外，我们提供了证据表明，在青春期，前列腺中存在胚胎起源、自我更新的组织驻留巨噬细胞和骨髓单核细胞来源的募集巨噬细胞共存。我们的研究结果表明，前列腺巨噬细胞促进神经模式形成，并可能进一步阐明我们对固有免疫系统在前列腺病理学中的作用的理解，包括对炎症和癌症的反应。
