Samelak-Czajka Anna, Marszałek-Zeńczak Małgorzata, Jackowiak Paulina
Pracownia Analiz Pojedynczych Komórek, Instytut Chemii Bioorganicznej Polskiej Akademii Nauk.
Postepy Biochem. 2024 Jul 1;70(2):190-203. doi: 10.18388/pb.2021_532.
Single-cell transcriptomics (scRNA-Seq) is a breakthrough technology that has opened the way to characterizing gene expression with unprecedented resolution. It has enabled the discovery of the cellular diversity of organisms and tracing their developmental processes. A range of technological solutions have been developed to allow analysis of tens of thousands to even a million cells in a single experiment, as well as an extensive set of tools for bioinformatics analysis of the generated data. The wealth of information provided by scRNA-Seq and the possibility of using this method to study cells, organoids, tissues and even entire organisms determine its wide range of applications. In this paper, we present the experimental and computational parts of the scRNA-Seq procedure, as well as the most important applications of this technology in biomedicine, developmental biology and plant biology.
单细胞转录组学（scRNA-Seq）是一项突破性技术，它以前所未有的分辨率开启了表征基因表达的道路。它使得发现生物体的细胞多样性并追踪其发育过程成为可能。一系列技术解决方案已经被开发出来，以便在单个实验中分析数万个甚至一百万个细胞，同时还有一套广泛的工具用于对所生成数据进行生物信息学分析。scRNA-Seq提供的丰富信息以及使用该方法研究细胞、类器官、组织甚至整个生物体的可能性决定了其广泛的应用范围。在本文中，我们介绍了scRNA-Seq程序的实验和计算部分，以及该技术在生物医学、发育生物学和植物生物学中的最重要应用。
