Rüfli Adrian
Pracownia Testów i Obrazowania Molekularnego, Instytut Chemii Bioorganicznej Polskiej Akademii Nauk, Poznań.
Postepy Biochem. 2024 Jul 1;70(2):139-149. doi: 10.18388/pb.2021_527.
Biological sciences are increasingly uncovering the foundations of life in greater detail, made possible by the development of research methods enabling exploration at the nanometer scale. Optical microscopy, a field with a significant contribution to current knowledge, is inherently limited by the Abbe limit, stemming from the fundamental wave properties of light. Through the efforts of scientists, this limit can be circumvented, as evidenced by STED and MINFLUX techniques. STED allows imaging with a resolution down to 40 nm, while MINFLUX enables resolution as fine as 2 nm. Both techniques require labelling of biological molecular targets with fluorescent markers and enable imaging in living cells, facilitating the study of dynamic biological processes. This article provides an introduction to super-resolution techniques STED and MINFLUX, demonstrating their utility through the example of studying kinesin movement along microtubules using the MINFLUX technique.
随着研究方法的发展，能够在纳米尺度上进行探索，生物科学正越来越详细地揭示生命的基础。光学显微镜对当前的知识有重大贡献，但由于光的基本波动特性，它固有地受到阿贝极限的限制。通过科学家们的努力，这一极限可以被规避，受激发射损耗显微镜（STED）和最小荧光通量显微镜（MINFLUX）技术就证明了这一点。STED能实现低至40纳米的分辨率成像，而MINFLUX能实现高达2纳米的分辨率。这两种技术都需要用荧光标记物标记生物分子靶点，并能在活细胞中成像，有助于研究动态生物过程。本文介绍了超分辨率技术STED和MINFLUX，并通过使用MINFLUX技术研究驱动蛋白沿微管运动的例子展示了它们的实用性。
