Key Laboratory of Coal Processing and Efficient Utilization, Ministry of Education, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China; Mechano Chemistry Research Institute, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China.
Key Laboratory of Coal Processing and Efficient Utilization, Ministry of Education, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China.
Ecotoxicol Environ Saf. 2024 Sep 15;283:116811. doi: 10.1016/j.ecoenv.2024.116811. Epub 2024 Jul 30.
In this work, the relationship and kinetics of biodegradation and bio-adsorption of benzo[a]pyrene (BaP) by Bacillus and Ascomycota were explored, and the metabolites of BaP under mixed microbial coculture were analyzed and characterized. The results show that BaP was removed through both biosorption and biodegradation. Under mixed microbial coculture, biosorption played a significant role in the early stage and biodegradation was predominant in the later stage. During the removal of BaP, the fungi exhibited remarkable adsorption capabilities for BaP with an adsorption efficiency (AE) of 38.14 %, while bacteria had a best degradation for BaP with a degradation efficiency (DE) of 56.13 %. Under the mixed microbial culture, the removal efficiency (RE) of BaP by the synergistic action of fungi and bacteria reached up to 76.12 % within 15 days. Kinetics analysis illustrated that the degradation and adsorption process of BaP were well fit to the first-order and the pseudo-second-order kinetic models, respectively. The research on the relationship between degradation and adsorption during microbial removal of BaP, as well as the synergistic effects of fungi and bacteria, will provide a theoretical guidance for two or even synthetic microbial communities.
在这项工作中，研究了苯并[a]芘（BaP）被芽孢杆菌和子囊菌生物降解和生物吸附的关系和动力学，并分析和表征了混合微生物共培养下 BaP 的代谢物。结果表明，BaP 通过生物吸附和生物降解两种方式被去除。在混合微生物共培养下，生物吸附在早期阶段起重要作用，而生物降解在后期阶段占主导地位。在 BaP 的去除过程中，真菌对 BaP 表现出显著的吸附能力，吸附效率（AE）为 38.14%，而细菌对 BaP 具有最佳的降解能力，降解效率（DE）为 56.13%。在混合微生物培养下，真菌和细菌的协同作用使 BaP 的去除效率（RE）在 15 天内达到了 76.12%。动力学分析表明，BaP 的降解和吸附过程分别很好地符合一级和拟二级动力学模型。这项关于微生物去除 BaP 过程中降解和吸附关系以及真菌和细菌协同作用的研究，将为两种甚至合成微生物群落提供理论指导。
