Department of Anesthesiology, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.
Department of Anesthesiology, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.
Clinics (Sao Paulo). 2024 Jul 30;79:100458. doi: 10.1016/j.clinsp.2024.100458. eCollection 2024.
The influences of Oxycodone (OXY) combined with Paclitaxel (PTX) on breast cancer cells are unclear. The present study aimed to examine the effects of OXY combined with PTX on the proliferation, apoptosis, and migration of human breast cancer SKBR3 cells and the underlying mechanism.
The proliferation, apoptosis and invasion of SKBR3 cells were assessed by CCK-8, colony formation assay, flowcytometric, Transwell assay and scratch assays, respectively. In addition, Western blotting was used to detect the expression of related proteins in these cells. The autophagic bodies were observed under a transmission electron microscope.
OXY (0.25, 0.5 and 1 mM) significantly inhibited the viability, colony-forming, migration, and invasion of SKBR3 cells as compared to the control group. Furthermore, OXY (0.25, 0.5 and 1 mM) markedly induced the apoptosis of SKBR3 cells and the levels of apoptosis-related proteins. In addition, OXY (0.25, 0.5 and 1 mM) and PTX inhibited the proliferation of SKBR3 cells synergistically as compared to PTX group in vitro. Moreover, OXY (0.25, 0.5 and 1 mM) significantly elevated the PTX-induced apoptosis in SKBR3 cells via downregulating the expression of N-cadherin, Becline-1 LC3-Ⅱ, p-Akt and p-mTOR and upregulating E-cadherin expression. Compared with the control group, OXY (1 mM) treatment induced autophagy in SKBR3 cells.
The present study indicates that OXY can enhance the antitumor effect of PTX on breast cancer in vitro. Hence, the combination of OXY with PTX may serve as a potential strategy for the treatment of breast cancer.
羟考酮（OXY）联合紫杉醇（PTX）对乳腺癌细胞的影响尚不清楚。本研究旨在探讨 OXY 联合 PTX 对人乳腺癌 SKBR3 细胞增殖、凋亡和迁移的影响及其机制。
采用 CCK-8 法、集落形成实验、流式细胞术、Transwell 实验和划痕实验分别检测 SKBR3 细胞的增殖、凋亡和迁移，Western blot 检测相关蛋白的表达，透射电镜观察自噬体。
与对照组相比，OXY（0.25、0.5 和 1mM）显著抑制 SKBR3 细胞的活力、集落形成、迁移和侵袭。此外，OXY（0.25、0.5 和 1mM）明显诱导 SKBR3 细胞凋亡及凋亡相关蛋白水平。另外，与 PTX 组相比，OXY（0.25、0.5 和 1mM）与 PTX 体外联合抑制 SKBR3 细胞增殖，协同作用更显著。此外，OXY（0.25、0.5 和 1mM）显著下调 N-钙黏蛋白、Becline-1 LC3-Ⅱ、p-Akt 和 p-mTOR 的表达，上调 E-钙黏蛋白的表达，增强了 PTX 诱导的 SKBR3 细胞凋亡。与对照组相比，OXY（1mM）处理诱导 SKBR3 细胞自噬。
本研究表明，OXY 可增强 PTX 对乳腺癌的体外抗肿瘤作用。因此，OXY 联合 PTX 可能成为治疗乳腺癌的一种潜在策略。
