College of Marine Science and Engineering, Nanjing Normal University, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing, 210023, China.
College of Marine Science and Engineering, Nanjing Normal University, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing, 210023, China; Co-Innovation Center for Marine Bio-Industry Technology, Lian Yungang, Jiangsu, 222005, China.
Fish Shellfish Immunol. 2024 Sep;152:109797. doi: 10.1016/j.fsi.2024.109797. Epub 2024 Jul 30.
Bacterial intestinal inflammation is a common disease of yellow catfish (Pelteobagrus fulvidraco) in high-density aquaculture. Understanding the interactions between host and intestinal bacteria is helpful to intestinal inflammatory disease control. Here, we constructed a model of intestinal inflammation after Aeromonas hydrophila infection in yellow catfish, and characterized variations in gene expression and microbiome in the gut through high-throughput sequencing. Furthermore, host gene-microbiome interactions were identified. Histology observation showed disordered distribution of columnar epithelial cells and decrease of goblet cells in intestine. A total of 4741 genes showed differentially expression, mostly in comparisons between 12 hpi group with each other groups respectively, including control, 24 hpi and 48 hpi groups. These genes were enriched in immune-related pathways including the IL-17 signaling pathway, triggering strong inflammatory response at the invading stage within 12 h. Subsequently, the host strengthened energy consumption by activating carbohydrate and lipid metabolism pathways to repair the intestinal mucosal immune defense line. In addition, fish with A. hydrophila infection show decreased richness of gut microbial, reduced relative abundance of probiotics including Akkermansia, and elevated pathogenic bacteria such as Plesimonas. An integrative analysis identified A. hydrophila-related genes, such as il22 and stat3, for which expression level is close associated with the shift of A. hydrophila-related bacteria relative abundance, such as Akkermansia and Cetobacterium. Aside from picturing the variations of intestine gene expression and mucosal microbiome of yellow catfish coping with A. hydrophila infection, our study probed the underlying host-microbe interactions in A. hydrophila infection induced intestinal inflammatory, providing new insights for disease control in aquaculture.
细菌性肠炎是高密度养殖黄颡鱼（Pelteobagrus fulvidraco）的常见病。了解宿主与肠道细菌的相互作用有助于控制肠道炎症性疾病。在这里，我们构建了黄颡鱼感染嗜水气单胞菌后的肠道炎症模型，并通过高通量测序对肠道中基因表达和微生物组的变化进行了特征分析。此外，还鉴定了宿主基因-微生物组的相互作用。组织学观察显示，肠道柱状上皮细胞排列紊乱，杯状细胞减少。共有 4741 个基因表现出差异表达，主要集中在 12 hpi 组与其他各组之间的比较中，包括对照组、24 hpi 组和 48 hpi 组。这些基因富集在免疫相关途径中，包括 IL-17 信号通路，在 12 小时的入侵阶段引发强烈的炎症反应。随后，宿主通过激活碳水化合物和脂质代谢途径来增强能量消耗，以修复肠道黏膜免疫防御线。此外，感染嗜水气单胞菌的鱼类肠道微生物丰富度降低，阿克曼氏菌等益生菌相对丰度降低，病原菌如普雷希莫纳菌等相对丰度增加。综合分析确定了与嗜水气单胞菌相关的基因，如 il22 和 stat3，其表达水平与阿克曼氏菌和 Cetobacterium 等与嗜水气单胞菌相关细菌的相对丰度变化密切相关。除了描绘黄颡鱼应对嗜水气单胞菌感染时肠道基因表达和黏膜微生物组的变化外，我们的研究还探讨了宿主-微生物相互作用在嗜水气单胞菌感染诱导的肠道炎症中的潜在机制，为水产养殖疾病控制提供了新的见解。
