Qinghai Provincial Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining 810008, China; Medical College, Qinghai University, Xining 810001, China.
Qinghai Provincial Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining 810008, China; CAS Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China; University of Chinese Academy of Sciences, 19(A) yuquan road, Beijing 10049, China.
Int J Biol Macromol. 2024 Oct;277(Pt 2):134281. doi: 10.1016/j.ijbiomac.2024.134281. Epub 2024 Jul 29.
Cordyceps sinensis, a traditionally prized medicinal fungus, contains polysaccharides as one of its main bioactive constituents, known for their significant immunomodulatory properties. In this study, we systematically investigated the composition and structure of Cordyceps sinensis polysaccharide, followed by an evaluation of its therapeutic effect on depression using a chronic restraint stress-induced depression model. The polysaccharide CSWP-2, extracted via hot water, precipitated with ethanol, and purified using DEAE-cellulose column chromatography from Cordyceps sinensis, is primarily composed of glucose, mannose, and galactose, with α-1,4-D-glucan as its major structural component. Behavioral tests, immunological profiling, metabolomics, and gut microbiota analyses indicated a notable ameliorative effect of CSWP-2 on depressive-like symptoms in mice. Furthermore, the action of CSWP-2 may be attributed to the modulation of the gut microbiome's abundance and its metabolic impacts, thereby transmitting signals to the host immune system and exerting immunomodulatory activity, ultimately contributing to its antidepressant effects.
蛹虫草，一种传统上备受推崇的药用真菌，含有多糖作为其主要生物活性成分之一，具有显著的免疫调节特性。在这项研究中，我们系统地研究了蛹虫草多糖的组成和结构，然后使用慢性束缚应激诱导的抑郁模型评估了其对抑郁症的治疗效果。多糖 CSWP-2 通过热水提取、乙醇沉淀和 DEAE-纤维素柱层析从蛹虫草中纯化得到，主要由葡萄糖、甘露糖和半乳糖组成，其主要结构成分为α-1,4-D-葡聚糖。行为测试、免疫学分析、代谢组学和肠道微生物组分析表明，CSWP-2 对小鼠的抑郁样症状有显著的改善作用。此外，CSWP-2 的作用可能归因于调节肠道微生物组的丰度及其代谢影响，从而向宿主免疫系统传递信号并发挥免疫调节活性，最终导致其抗抑郁作用。
