Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, China.
Institute of Agricultural Genome, Chinese Academy of Agricultural Sciences (Shenzhen), Shenzhen Branch of Lingnan Modern Agricultural Science and Technology Laboratory, Key Laboratory of Agricultural Gene Data Analysis, Ministry of Agriculture and Rural Affairs, Shenzhen 518120, China; Northeast Forestry University, Key Laboratory of Sustainable Management of Forest Ecosystem, Ministry of Education, Harbin 150040, China.
Pestic Biochem Physiol. 2024 Aug;203:105998. doi: 10.1016/j.pestbp.2024.105998. Epub 2024 Jun 21.
Recognition of sex pheromones released by heterosexual moths via sex pheromone receptors is key for establishing mating connections in moths. The day-flying moth Phauda flammans is an oligophagous pest in southern cities of China and Southeast Asian countries. Our previous study reported that male P. flammans can be attracted to two sex pheromone compounds [Z-9-hexadecenal and (Z, Z, Z)-9,12,15-octadecadienal] released by females in the field; however, the mechanism of olfactory recognition is not clear. In this study, two sex pheromone receptor genes (PflaOR29 and PflaOR44) were cloned. Among the different tissues, both PflaOR29 and PflaOR44 were highly expressed in the antennae of mated male adults. At different developmental stages, the expression levels of PflaOR29 and PflaOR44 were significantly greater in mated male adults than other stages. The fluorescence signals of PflaOR29 and PflaOR44 were mostly distributed on the dorsal side of the antennae, with a large number of trichoid sensilla. The results of the gene function of PflaOR29 and PflaOR44 based on a Drosophila empty neuron heterologous expression system indicated that PflaOR29 strongly responded to (Z, Z, Z)-9,12,15-octadecadienal but not to Z-9-hexadecenal, whereas PflaOR44 did not respond to the two sex pheromones. Our findings clarify the sex pheromone receptor gene corresponding to (Z, Z, Z)-9,12,15-octadecatrienal. These results provide essential information for analyzing the mechanism of sexual communication in diurnal moths and for identifying target genes for the development of efficient attractants.
异性蛾释放的性信息素通过性信息素受体被识别，这是蛾类建立交配联系的关键。昼行性飞蛾 Phauda flammans 是中国南方城市和东南亚国家的一种寡食性害虫。我们之前的研究报告称，雄性 P. flammans 可以被雌性在野外释放的两种性信息素化合物[Z-9-十六碳烯醛和(Z, Z, Z)-9,12,15-十八碳三烯醛]所吸引；然而，嗅觉识别的机制尚不清楚。在这项研究中，我们克隆了两个性信息素受体基因(PflaOR29 和 PflaOR44)。在不同的组织中，PflaOR29 和 PflaOR44 在交配雄性成虫的触角中都有高度表达。在不同的发育阶段，交配雄性成虫中 PflaOR29 和 PflaOR44 的表达水平显著高于其他阶段。PflaOR29 和 PflaOR44 的荧光信号主要分布在触角的背面，有大量的毛形感觉器。基于果蝇空神经元异源表达系统的 PflaOR29 和 PflaOR44 的基因功能结果表明，PflaOR29 对(Z, Z, Z)-9,12,15-十八碳三烯醛有强烈的反应，但对 Z-9-十六碳烯醛没有反应，而 PflaOR44 对这两种性信息素都没有反应。我们的发现阐明了与(Z, Z, Z)-9,12,15-十八碳三烯醛对应的性信息素受体基因。这些结果为分析昼行性飞蛾性通讯机制以及识别高效引诱剂的靶基因提供了重要信息。
