分子水平研究 P450 CYP321A7 基因在斜纹夜蛾解毒过程中的功能及其在 lambda-氯氰菊酯解毒中的作用。
Molecular insights into the functional analysis of P450 CYP321A7 gene in the involvement of detoxification of lambda-cyhalothrin in Spodoptera frugiperda.
机构信息
Guangdong Engineering Technology Research Centre of Modern Eco-agriculture and Circular Agriculture, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China; Heyuan Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Heyuan 517000, China; Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China.
Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA; USDA-ARS Horticultural Crops Research Unit, 3420 NW Orchard Avenue, Corvallis, OR 97330, USA.
出版信息
Pestic Biochem Physiol. 2024 Aug;203:106009. doi: 10.1016/j.pestbp.2024.106009. Epub 2024 Jul 2.
Fall armyworm, Spodoptera frugiperda (J. E. Smith), is a widely recognized global agricultural pest that has significantly reduced crop yields all over the world. S. frugiperda has developed resistance to various insecticides. Insect cytochrome P450 monooxygenases (CYPs or P450s) play an important role in detoxifying insecticides, leading to increased resistance in insect populations. However, the function of the specific P450 gene for lambda-cyhalothrin resistance in S. frugiperda was unclear. Herein, the expression patterns of 40 P450 genes in the susceptible and lambda-cyhalothrin-resistant populations were analyzed. Among them, CYP321A7 was found to be overexpressed in the resistant population, specifically LRS (resistance ratio = 25.38-fold) derived from a lambda-cyhalothrin-susceptible (SS) population and FLRS (a population caught from a field, resistance ratio = 63.80-fold). Elevated enzyme activity of cytochrome P450 monooxygenases (P450s) was observed for LRS (2.76-fold) and the FLRS (4.88-fold) as compared to SS, while no significant differences were observed in the activities of glutathione S-transferases and esterases. Furthermore, the knockdown of CYP321A7 gene by RNA interference significantly increased the susceptibility to lambda-cyhalothrin. Remarkably, the knockdown of CYP321A7 reduced the enzymatic activity of P450 by 43.7%, 31.9%, and 22.5% in SS, LRS, and FLRS populations, respectively. Interestingly, fourth-instar larvae treated with lambda-cyhalothrin at the LC dosage had a greater mortality rate due to RNA interference-induced suppression of CYP321A7 (with increases of 61.1%, 50.0%, and 45.6% for SS, LRS, and FLRS populations, respectively). These findings suggest a link between lambda-cyhalothrin resistance and continual overexpression of CYP321A7 in S. frugiperda larvae, emphasizing the possible importance of CYP321A7 in lambda-cyhalothrin detoxification in S. frugiperda.
草地贪夜蛾，Spodoptera frugiperda（J. E. Smith），是一种广泛认可的全球农业害虫，它已大大降低了世界各地的作物产量。S. frugiperda 已经对各种杀虫剂产生了抗性。昆虫细胞色素 P450 单加氧酶（CYPs 或 P450s）在解毒杀虫剂方面发挥着重要作用，导致昆虫种群的抗性增加。然而，S. frugiperda 对 lambda-氯氰菊酯抗性的特定 P450 基因的功能尚不清楚。在此，分析了敏感和 lambda-氯氰菊酯抗性种群中 40 个 P450 基因的表达模式。其中，在抗性种群中发现 CYP321A7 过度表达，特别是从 lambda-氯氰菊酯敏感（SS）种群衍生的 LRS（抗性比= 25.38 倍）和 FLRS（从田间捕获的种群，抗性比= 63.80 倍）。与 SS 相比，LRS（2.76 倍）和 FLRS（4.88 倍）中细胞色素 P450 单加氧酶（P450s）的酶活性升高，而谷胱甘肽 S-转移酶和酯酶的活性没有明显差异。此外，通过 RNA 干扰敲低 CYP321A7 基因显著增加了对 lambda-氯氰菊酯的敏感性。值得注意的是，在 SS、LRS 和 FLRS 种群中，CYP321A7 基因的敲低分别使 P450 的酶活性降低了 43.7%、31.9%和 22.5%。有趣的是，用 lambda-氯氰菊酯在 LC 剂量处理四龄幼虫后，由于 RNA 干扰诱导的 CYP321A7 抑制，死亡率更高（SS、LRS 和 FLRS 种群的死亡率分别增加了 61.1%、50.0%和 45.6%）。这些发现表明，S. frugiperda 幼虫中 lambda-氯氰菊酯抗性与 CYP321A7 的持续过度表达之间存在联系，强调了 CYP321A7 在 S. frugiperda 中 lambda-氯氰菊酯解毒中的可能重要性。
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