Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Beijing 100097, China.
National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, 350002, China.
Pestic Biochem Physiol. 2024 Aug;203:105987. doi: 10.1016/j.pestbp.2024.105987. Epub 2024 Jun 13.
Bemisia tabaci is one of the most destructive agricultural insect pests around the world, and it has developed high levels of resistance to most pesticides. Dimpropyridaz, a novel insecticide developed by BASF, displays excellent activity against piercing-sucking insect pests. In this study, baseline of susceptibility showed all tested field populations of B. tabaci are susceptible to dimpropyridaz. After continuous selection with dimpropyridaz in the lab, a B. tabaci strain (F12) developed 2.2-fold higher level of resistance compared with a susceptible MED-S strain, and the realized heritability (h) was estimated as 0.0518. The F12 strain displayed little cross-resistance to afidopyropen, cyantraniliprole, sulfoxaflor, or abamectin, and significantly increased activity of cytochrome P450 monooxygenase (P450). The fitness cost of dimpropyridaz resistance was evident in F12 strain, which had a relative fitness of 0.95 and significantly lower fecundity per female compared with MED-S strain. Taken together, B. tabaci displays high susceptibility to dimpropyridaz in the field, and low risk of developing resistance to dimpropyridaz under successive selection pressure. Little cross-resistance to popular insecticides was found, and fitness cost associated dimpropyridaz resistance was observed. Higher activity of cytochrome P450 in the F12 strain, may be involved in the process of detoxifying dimpropyridaz in whitefly.
烟粉虱是世界上最具破坏性的农业害虫之一，对大多数杀虫剂都产生了高水平的抗性。巴斯夫公司开发的新型杀虫剂双丙环虫酯对刺吸式害虫具有优异的活性。本研究中，敏感性基线表明，所有测试的烟粉虱田间种群均对双丙环虫酯敏感。在实验室中连续用双丙环虫酯选择后，与敏感 MED-S 株相比，烟粉虱种群（F12 株）产生了 2.2 倍的抗性水平，实现遗传力（h）估计为 0.0518。F12 株对阿维菌素、氯虫苯甲酰胺、螺虫乙酯或噻虫胺的交叉抗性很小，但细胞色素 P450 单加氧酶（P450）的活性显著增加。双丙环虫酯抗性的适合度代价在 F12 株中明显，其相对适合度为 0.95，与 MED-S 株相比，每头雌虫的繁殖力显著降低。总之，烟粉虱田间对双丙环虫酯高度敏感，在连续选择压力下，对双丙环虫酯产生抗性的风险较低。对常用杀虫剂的交叉抗性很小，并且观察到与双丙环虫酯抗性相关的适合度代价。F12 株细胞色素 P450 活性较高，可能参与了烟粉虱对双丙环虫酯解毒的过程。
