Key Laboratory of Land Resources Evaluation and Monitoring in Southwest, Ministry Education of China, Chengdu, Sichuan 610066, China; College of Life Sciences, Sichuan Normal University, Chengdu, Sichuan 610066, China.
Key Laboratory of Land Resources Evaluation and Monitoring in Southwest, Ministry Education of China, Chengdu, Sichuan 610066, China; College of Life Sciences, Sichuan Normal University, Chengdu, Sichuan 610066, China.
Int J Biol Macromol. 2024 Sep;276(Pt 2):133785. doi: 10.1016/j.ijbiomac.2024.133785. Epub 2024 Jul 31.
Because of the poor stability and rheological properties of fat-free stirred yogurt fortified with fruit pulp, new functional polysaccharides as a natural emulsifier, which can increase viscosity in the aqueous phase, may be needed. This study aimed to evaluate the effects of Exidia yadongensis polysaccharide (EYP) as emulsifier on the stability, aroma, and antioxidant activities of mango buffalo yogurt at 4 °C for 25 days. The yogurt with 15 g/L EYP gave a higher content of 215 g/L total solids, 11.3 g/L exopolysaccharides, 0.10 g/L total polyphenols, 630.5 g/L water-holding capacity, and 11.43 g/kg total free amino acids, and maintained better texture, DPPH scavenging activity of 54.05 % and OH scavenging rates of 67.16 %. Moreover, the EYP exhibited the expected ability to weaken postacidification, syneresis, and growth of microorganism, and greatly promote the textural, rheological properties, suspension stability, microstructure, and aroma profiles of stirred mango-flavored buffalo yogurt (p < 0.05). In addition, the addition of 15 g/L EYP can inhibit protein degradation and improve the stability of secondary structure of the protein complex in mango yogurt during 25 days of storage. Therefore, EYP (15 g/L) could be used as natural positive functional factors and emulsifiers in such fat-free stirred yogurt industry.
由于无脂搅拌型酸乳添加果浆后稳定性和流变性较差,需要使用新型功能性多糖作为天然乳化剂,这种多糖可以增加水相的黏度。本研究旨在评估黄韧伞多糖(EYP)作为乳化剂对芒果水牛搅拌型酸奶在 4℃下 25 天的稳定性、香气和抗氧化活性的影响。在酸奶中添加 15 g/L 的 EYP 可以使其总固形物含量提高到 215 g/L,胞外多糖含量提高到 11.3 g/L,总多酚含量提高到 0.10 g/L,持水力提高到 630.5 g/L,游离氨基酸总量提高到 11.43 g/kg,并保持更好的质地,DPPH 清除活性为 54.05%,OH 清除率为 67.16%。此外,EYP 具有预期的减弱后酸化、乳清析出和微生物生长的能力,极大地促进了搅拌型芒果风味水牛酸奶的质构、流变特性、悬浮稳定性、微观结构和香气特征(p<0.05)。此外,添加 15 g/L 的 EYP 可以抑制蛋白质降解,提高芒果酸奶中蛋白质复合结构二级结构的稳定性,在 25 天的贮藏期内。因此,EYP(15 g/L)可以作为无脂搅拌型酸奶中天然的积极功能因子和乳化剂。