Narayanan R B, Ramu G, Malaviya G N, Sengupta U, Desikan K V
Indian J Lepr. 1985 Apr-Jun;57(2):265-72.
A study was made on the in situ characteristics of dermal infiltrates in the early and late lepromin reaction with monoclonal antibodies defining T cell subsets, Langerhan cells and Ia like antigens. The early reaction (24 hrs) was elicited either with standard Dharmendra lepromin or leprosin-A and the late reaction (3-4 weeks) was elicited with standard Dharmendra lepromin. In all, 15 biopsies were studied. Most lymphocytes in the infiltrates of both the lepromin and leprosin reactions were positive for OKT 11, Leu 3a, OKT 8 and Ia like antigens indicating thereby the presence of activated T cells. A high proportion of OKT6 + cells were also noticed in the infiltrates of these reactions. In the late reaction, the lymphocytes in the granulomas were predominantly activated T lymphocytes expressing OKT 11, Leu 3a, OKT 8 and Ia like antigens. Leu 3a + cells were scattered diffusely amidst the epithelioid cells. In contrast, OKT 8 + cells were present mainly in the peripheral region of the granuloma. A small proportion of OKT6 + cells were also seen in these granulomas. Ia like antigens and T6 antigens were not discernible on the epithelioid cells. No difference in the number of OKT6 + epidermal langerhan cells was observed in the various types of reactions.
利用定义T细胞亚群、朗格汉斯细胞和Ia样抗原的单克隆抗体，对麻风菌素反应早期和晚期真皮浸润的原位特征进行了研究。早期反应（24小时）用标准达曼德拉麻风菌素或麻风素A激发，晚期反应（3 - 4周）用标准达曼德拉麻风菌素激发。总共研究了15份活检标本。麻风菌素和麻风素反应浸润中的大多数淋巴细胞对OKT 11、Leu 3a、OKT 8和Ia样抗原呈阳性，从而表明存在活化的T细胞。在这些反应的浸润中也发现高比例的OKT6 +细胞。在晚期反应中，肉芽肿中的淋巴细胞主要是表达OKT 11、Leu 3a、OKT 8和Ia样抗原的活化T淋巴细胞。Leu 3a +细胞分散在上皮样细胞之间。相比之下，OKT 8 +细胞主要存在于肉芽肿的周边区域。在这些肉芽肿中也可见一小部分OKT6 +细胞。在上皮样细胞上未观察到Ia样抗原和T6抗原。在各种类型的反应中，OKT6 +表皮朗格汉斯细胞的数量没有差异。
