Hawai'i Institute of Marine Biology, School of Ocean and Earth Science and Technology, University of Hawaii at Mānoa, Kāne'ohe, Hawai'i, USA.
Bishop Museum, Honolulu, Hawai'i, USA.
J Fish Biol. 2024 Oct;105(4):1256-1267. doi: 10.1111/jfb.15893. Epub 2024 Jul 31.
A size-based, histological analysis of the reproductive life history of the blacktip grouper, Epinephelus fasciatus (Forsskål 1775), was conducted in Indonesia to evaluate the error rate associated with macroscopic reproductive analysis. Histological results indicated that E. fasciatus was protogynous with female L at 13.4 cm total length (L) and a size at sexual transition of 22.0 cm L. The weight-length relationship for the species was W = 0.011 L. Overall sex ratios were significantly female biased, operational sex ratios were significantly male biased, and sex ratios of mature individuals varied predictably with length from female to male dominance as size increased. No significant relationship between length and batch fecundity was found. The population has a spawning period from February to August. Overall, 54.4% of macroscopic evaluations were incorrect compared to histological results. Of the errors, 14.8% were a failure to detect ovotestes, 12.7% were classifying non-gonadal tissue as ovary or testis, 12.2% were misclassifying sex, and 12.7% were misclassifying maturity status. However, the largest source of error (47.7%) was from misclassifying both sex and maturity status. Of these, 92.9% were macroscopically classified as immature females, but were histologically confirmed to be mature males. Compared to histological results, the only accurate macroscopic results were the absence of a sex-based difference in weight-length relationship and spawning seasonality estimated by a gonadosomatic index (February-June). The use of macroscopic methods to estimate reproductive life-history parameters for sex-changing reefes fish may introduce significant inaccuracies and misinterpretations. Of the parameters estimated by histological methods, size at maturity, size-specific sex ratios, and spawning seasonality have the greatest potential to inform local fishery management policy.
对印度尼西亚的黑鳍石斑鱼（Epinephelus fasciatus（Forsskål 1775））进行了基于大小的组织学生殖史分析，以评估与宏观生殖分析相关的错误率。组织学结果表明，黑鳍石斑鱼为雌先型性转变鱼类，性转变时的最小雌鱼全长（L）为 13.4cm，性转变时全长（L）为 22.0cm。该物种的体重-体长关系为 W = 0.011L。总体性别比例明显偏向雌性，性操作比明显偏向雄性，成熟个体的性别比例随着体长的增加从雌性优势变为雄性优势，可预测地发生变化。未发现长度与批量繁殖力之间存在显著关系。该种群的繁殖期为 2 月至 8 月。总体而言，与组织学结果相比，有 54.4%的宏观评估结果是不正确的。在这些错误中，有 14.8%是未能检测到卵黄卵巢，12.7%是将非性腺组织归类为卵巢或睾丸，12.2%是性别分类错误，12.7%是成熟状态分类错误。然而，最大的错误来源（47.7%）是同时错误地分类性别和成熟状态。其中，92.9%的个体在宏观上被归类为未成熟的雌性，但在组织学上被确认为成熟的雄性。与组织学结果相比，唯一准确的宏观结果是性腺指数（2 月至 6 月）估计的体重-体长关系和繁殖季节性没有性别差异。使用宏观方法估计性转变珊瑚鱼的生殖生活史参数可能会引入重大的不准确和误解。在组织学方法估计的参数中，成熟大小、大小特异性性别比例和繁殖季节性最有可能为当地渔业管理政策提供信息。
