Jafar Sameri Maryam, Savari Feryal, Mard Seyyed Ali, Rezaie Anahita, Kalantar Mojtaba
Department of physiology, medicine faculty, Abadan University of Medical Sciences, Abadan, Iran.
Department of Medical Basic Sciences, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.
Rep Biochem Mol Biol. 2024 Jan;12(4):619-630. doi: 10.61186/rbmb.12.4.619.
Organ ischemia-reperfusion (IR) is a common clinical condition associated with various situations such as trauma surgery, organ transplantation, and myocardial ischemia. Current therapeutic methods for IR injury have limitations, and nanotechnology, particularly zinc oxide nanoparticles (ZnO NPs), offers new approaches for disease diagnosis and treatment. In this study, we investigated the protective and anti-apoptotic effects of ZnO NPs in liver ischemia-reperfusion (IR) injury in rats.
Forty-eight male rats were divided into six groups: sham, ZnO5, ZnO10, ischemia-reperfusion (IR), IR+ZnO5, and IR+ZnO10. The protective effect of ZnO NPs was evaluated by liver enzymes (AST, ALT, Bilirubin, ALP), biochemical (TAC, TNF-α, and MDA), molecular examinations (Bcl2, BAX), and histopathological evaluations (H&E, TUNEL).
Pre-treatment with ZnO5 and ZnO10 improved hepatic function in IR liver injury, attenuated the levels of oxidants (P = 0.03) and inflammatory mediators, and reduced apoptosis (P = 0). ZnO10 was found to have a greater effect on ischemic reperfusion injury than ZnO5 did. Histopathological examination also showed a dose-dependent decrease in alterations in the IR+ZnO5 and IR+ZnO10 groups.
Administration of ZnO5 and ZnO10 improved liver function after IR. The findings of this study suggest that ZnO NPs have a protective effect against oxidative stress and apoptosis in liver ischemia-reperfusion injury in rats. These results may have important implications for developing advanced methods in ischemia-reperfusion treatment.
器官缺血再灌注（IR）是一种常见的临床情况，与创伤手术、器官移植和心肌缺血等多种情况相关。目前针对IR损伤的治疗方法存在局限性，而纳米技术，特别是氧化锌纳米颗粒（ZnO NPs），为疾病的诊断和治疗提供了新途径。在本研究中，我们研究了ZnO NPs对大鼠肝脏缺血再灌注（IR）损伤的保护和抗凋亡作用。
将48只雄性大鼠分为六组：假手术组、ZnO5组、ZnO10组、缺血再灌注（IR）组、IR + ZnO5组和IR + ZnO10组。通过肝酶（AST、ALT、胆红素、碱性磷酸酶）、生化指标（总抗氧化能力、肿瘤坏死因子-α和丙二醛）、分子检测（Bcl2、BAX）和组织病理学评估（苏木精-伊红染色、TUNEL）来评估ZnO NPs的保护作用。
ZnO5和ZnO10预处理改善了IR肝损伤中的肝功能，降低了氧化剂水平（P = 0.03）和炎症介质水平，并减少了细胞凋亡（P = 0）。发现ZnO10对缺血再灌注损伤的作用比ZnO5更大。组织病理学检查还显示，IR + ZnO5组和IR + ZnO10组的改变呈剂量依赖性降低。
给予ZnO5和ZnO10可改善IR后的肝功能。本研究结果表明，ZnO NPs对大鼠肝脏缺血再灌注损伤中的氧化应激和细胞凋亡具有保护作用。这些结果可能对开发缺血再灌注治疗的先进方法具有重要意义。
