Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER) - Raebareli, Transit Campus, Bijnour-Sisendi Road, Sarojini Nagar, Lucknow-226002, Uttar Pradesh, India.
Food Funct. 2024 Aug 27;15(17):8712-8728. doi: 10.1039/d4fo02602k.
: The aim of the present study was to study the potential therapeutic effects of quercetin in protection against repeated intranasal exposure of an amyloid-beta-induced mouse model. : Mice received intranasal Aβ (5 μg/10 μL) exposure once daily for seven consecutive days. Quercetin was orally administered to them at 30 mg kg and 100 mg kg doses for one week starting from day five following Aβ peptide administration. Following this, the animals were evaluated for neurobehavioral parameters using a Morris water maze test and a novel object recognition test. Further to this, the biomarkers for neuroinflammation and neurodegeneration were evaluated in the hippocampus and cortex regions of the brain in these animals. : Multiple exposures to intranasal Aβ led to a significant decline in the learning and cognitive memory of the animals, whereas oral treatment with quercetin at dosages of 30 and 100 mg kg alleviated Aβ-induced effects. Quercetin treatment significantly reduced Aβ accumulation, oxidative stress and proinflammatory cytokine biomarkers in the brain. In addition, it also alleviated the activation of astrocytic biomarkers, amyloid precursor protein and phosphorylated-tau proteins in the brain. : Quercetin was found to be a potent antioxidant, anti-inflammatory compound with protection against neurodegenerative damage and improved learning and cognitive memory in a repeated Aβ-exposure model of AD.
本研究旨在研究槲皮素在预防重复鼻内给予淀粉样蛋白-β诱导的小鼠模型中的潜在治疗作用。
小鼠每天接受一次鼻内 Aβ（5μg/10μL）暴露，连续 7 天。从 Aβ肽给药后的第五天开始，用 30mg/kg 和 100mg/kg 的剂量对它们进行一周的口服给药。之后，通过 Morris 水迷宫测试和新物体识别测试对动物进行神经行为参数评估。此外，还评估了这些动物大脑海马区和皮质区的神经炎症和神经退行性变生物标志物。
多次鼻内给予 Aβ导致动物的学习和认知记忆明显下降，而用 30mg/kg 和 100mg/kg 的剂量口服给予槲皮素可减轻 Aβ诱导的作用。槲皮素治疗显著降低了大脑中的 Aβ 积累、氧化应激和促炎细胞因子生物标志物。此外，它还减轻了大脑中星形胶质细胞生物标志物、淀粉样前体蛋白和磷酸化 tau 蛋白的激活。
槲皮素被发现是一种有效的抗氧化剂、抗炎化合物，可防止神经退行性损伤，并改善 AD 中重复 Aβ 暴露模型的学习和认知记忆。
