Department of Anatomy, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Department of Basic Medical Sciences, College of Medicine, Prince Sattam Bin Abdulaziz University, Al- Kharj, Kingdom of Saudi Arabia.
Ultrastruct Pathol. 2024 Sep 2;48(5):338-350. doi: 10.1080/01913123.2024.2386009. Epub 2024 Aug 1.
Diabetes mellitus is a common metabolic disorder. It is associated with serious life-threatening complications if not properly managed. The current study aimed at investigating the possible protective role of propolis on streptozotocin-induced diabetic nephropathy. A diabetic rat model was induced by a single intraperitoneal injection of 55 mg/kg streptozotocin. After 4 days, the diabetic rats received oral propolis (300 mg/kg/day) via gastric gavage for 28 days. Biochemical, histopathological and ultrastructural evaluations were performed. The results showed that: streptozotocin-induced diabetes was associated with a marked decrease in the serum high-density lipoproteins and antioxidant enzymes. However, a significant elevation in the levels of serum creatinine, blood urea nitrogen, uric acid, cholesterol, triglycerides and low-density lipoproteins was detected. Furthermore, streptozotocin treatment induced histopathological alterations of the renal cortex; in the form of distorted glomerular capillaries, widened Bowman's space and signs of epithelial tubular degeneration. Ultra-structurally, thickening and irregularity of the glomerular basement membrane and podocytes foot processes effacement were observed. The tubular epithelial cells showed swollen vacuolated mitochondria, scarce basal infoldings and loss of microvilli. Conversely, propolis partially restored the normal lipid profile, antioxidant biomarkers and renal cortical morphology. Propolis exhibited a sort of renoprotection through hypoglycemic, anti-hyperlipidemic and antioxidant effects.
糖尿病是一种常见的代谢紊乱疾病。如果不加以适当治疗，它会导致严重的危及生命的并发症。本研究旨在探讨蜂胶对链脲佐菌素诱导的糖尿病肾病的可能保护作用。通过单次腹腔注射 55mg/kg 链脲佐菌素诱导糖尿病大鼠模型。4 天后，糖尿病大鼠通过胃灌胃给予 300mg/kg/天的蜂胶 28 天。进行了生化、组织病理学和超微结构评估。结果表明：链脲佐菌素诱导的糖尿病与血清高密度脂蛋白和抗氧化酶显著降低有关。然而，血清肌酐、血尿素氮、尿酸、胆固醇、甘油三酯和低密度脂蛋白水平显著升高。此外，链脲佐菌素处理诱导了肾皮质的组织病理学改变；表现为肾小球毛细血管扭曲、鲍曼氏腔增宽和上皮管状退化的迹象。超微结构上，观察到肾小球基底膜和足细胞突起的增厚和不规则以及微绒毛的消失。肾小管上皮细胞显示肿胀的空泡化线粒体、稀少的基底内褶和微绒毛的丧失。相反，蜂胶部分恢复了正常的脂质谱、抗氧化生物标志物和肾皮质形态。蜂胶通过降血糖、抗高血脂和抗氧化作用表现出一定的肾脏保护作用。
