Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115 Calabar, Cross River State, Nigeria.
Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.
Molecules. 2021 Jun 5;26(11):3441. doi: 10.3390/molecules26113441.
Diabetic nephropathy is reported to occur as a result of the interactions between several pathophysiological disturbances, as well as renal oxidative stress and inflammation. We examined the effect of Malaysian propolis (MP), which has anti-hyperglycemic, antioxidant and anti-inflammatory properties, on diabetes-induced nephropathy. Diabetic rats were either treated with distilled water (diabetic control (DC) group), MP (300 mg/kg b.w./day), metformin (300 mg/kg b.w./day) or MP + metformin for four weeks. We found significant increases in serum creatinine, urea and uric acid levels, decreases in serum sodium and chloride levels, and increase in kidney lactate dehydrogenase activity in DC group. Furthermore, malondialdehyde level increased significantly, while kidney antioxidant enzymes activities, glutathione level and total antioxidant capacity decreased significantly in DC group. Similarly, kidney immunoexpression of nuclear factor kappa B, tumor necrosis factor-, interleukin (IL)-1β and caspase-3 increased significantly, while IL-10 immunoexpression decreased significantly in DC group relative to normal control group. Histopathological observations for DC group corroborated the biochemical data. Intervention with MP, metformin or both significantly mitigated these effects and improved renal function, with the best outcome following the combined therapy. MP attenuates diabetic nephropathy and exhibits combined beneficial effect with metformin.
糖尿病肾病据报道是由于几种病理生理紊乱、肾氧化应激和炎症相互作用而发生的。我们研究了具有降血糖、抗氧化和抗炎特性的马来西亚蜂胶（MP）对糖尿病肾病的影响。糖尿病大鼠分别用蒸馏水（糖尿病对照组（DC）组）、MP（300mg/kg bw/天）、二甲双胍（300mg/kg bw/天）或 MP+二甲双胍治疗四周。我们发现 DC 组血清肌酐、尿素和尿酸水平显著升高，血清钠和氯水平降低，肾乳酸脱氢酶活性升高。此外，DC 组丙二醛水平显著升高，而肾抗氧化酶活性、谷胱甘肽水平和总抗氧化能力显著降低。同样，DC 组肾核因子 kappa B、肿瘤坏死因子-α、白细胞介素（IL）-1β和半胱天冬酶-3的免疫表达显著增加，而 IL-10 的免疫表达显著降低。DC 组的组织病理学观察结果与生化数据相符。MP、二甲双胍或两者联合干预显著减轻了这些影响，改善了肾功能，联合治疗的效果最佳。MP 可减轻糖尿病肾病，并与二甲双胍具有联合的有益作用。
